Vascular Endothelial Growth Factor, Rat Active Protein | VEGFA active protein

Vascular Endothelial Growth Factor, Recombinant, Rat (VEGF)

Cat. Num. AAA650556

• Gene Names: VEGFA; VPF; VEGF; MVCD1; MGC70609
• Purity: Highly Purified; ~95% (SDS-PAGE). Purified by Ion Exchange chromatography.
• Synonyms: Vascular Endothelial Growth Factor; Rat; Recombinant; Rat (VEGF); VEGFA active protein

• Purity/Purification: Highly Purified; ~95% (SDS-PAGE). Purified by Ion Exchange chromatography.
• Form/Format: Supplied as a lyophilized powder. BSA free. Reconstitute with sterile, ddH2O or buffer, 0.1% BSA. to a concentration of 0.1-1mg/ml.

• Biological Activity: ED50 range=10-100ng/ml, determined by the dose dependent proliferation of human umbilical vein cells (HUVEC).
• Endotoxin Level: 0.1ng/ug
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O or buffer, 0.1% BSA., aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for VEGFA active protein

VEGF (Vascular Endothelial Growth Factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeablility inducing properties specific for the vascular endothelium. Multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues have been described which arise from alternative splicing of a single gene. The smaller two isoforms, VEGF 165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell-associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

Product Categories/Family for VEGFA active protein

Growth Factors; Cytokines; Growth Factors-VEGF

NCBI and Uniprot Product Information

• NCBI GI #: 181971
• NCBI GeneID: 7422
• UniProt Accession #: P15692; O60720; O75875; Q074Z4; Q16889; Q5UB46; Q6P0P5; Q96KJ0; Q96L82; Q96NW5; Q9H1W8; B5BU86; Q59FH5; Q6WZM0; Q71S09; Q96FD9; Q9UNS8
• Molecular Weight: 27,042 Da
• NCBI Official Full Name: vascular endothelial growth factor
• NCBI Official Synonym Full Names: vascular endothelial growth factor A
• NCBI Official Symbol: VEGFA
• NCBI Official Synonym Symbols: VPF; VEGF; MVCD1; MGC70609
• NCBI Protein Information: vascular endothelial growth factor A; OTTHUMP00000016487; OTTHUMP00000016488; OTTHUMP00000165985; OTTHUMP00000165986; OTTHUMP00000165987; OTTHUMP00000224107; OTTHUMP00000224108; OTTHUMP00000224109; OTTHUMP00000224153; OTTHUMP00000224154; OTTHUMP00000224423; OTTHUMP00000224424; OTTHUMP00000224425; OTTHUMP00000224426; OTTHUMP00000224427; OTTHUMP00000224428; OTTHUMP00000224429; OTTHUMP00000224430; vascular permeability factor
• UniProt Protein Name: Vascular endothelial growth factor A
• UniProt Gene Name: VEGFA
• UniProt Synonym Gene Names: VEGF
• UniProt Entry Name: VEGFA_HUMAN

NCBI Description

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq]

Uniprot Description

Function: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Ref.27 Ref.30

Subunit structure: Homodimer; disulfide-linked. Also found as heterodimer with PGF

Subcellular location: Secreted. Note: VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. Ref.26 Ref.28 Ref.32

Tissue specificity: Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.

Induction: Regulated by growth factors, cytokines, gonadotropins, nitric oxide, hypoxia, hypoglycemia and oncogenic mutations.

Involvement in disease: Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [

MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.

Sequence similarities: Belongs to the PDGF/VEGF growth factor family.

Sequence caution: The sequence AAC63102.1 differs from that shown. Reason: Erroneous initiation. The sequence AAC63143.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.The sequence CAC19512.2 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.The sequence CAC19516.2 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.