Transforming Growth Factor beta2 Active Protein | TGFB2 active protein

Transforming Growth Factor beta2, Recombinant, Human (TGFb2, Polyergin, Cetermin, G-TSF, Glioblastoma-derived T-cell suppressor factor, BSC-1 cell growth inhibitor)

Cat. Num. AAA650502

• Gene Names: TGFB2; MGC116892; TGF-beta2
• Purity: Highly Purified; ~98% (SDS-PAGE, HPLC). Purified by sequential chromatography.
• Synonyms: Transforming Growth Factor beta2; Recombinant; Human (TGFb2; Polyergin; Cetermin; G-TSF; Glioblastoma-derived T-cell suppressor factor; BSC-1 cell growth inhibitor); TGFB2 active protein

• Purity/Purification: Highly Purified; ~98% (SDS-PAGE, HPLC). Purified by sequential chromatography.
• Form/Format: Supplied as a lyophilized powder from sodium citrate buffer, pH 3.5. Sterile filtered prior to lyophilization. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml. Do not vortex.

• Biological Activity: ED50 0.2ng/ml (5x10e6units/mg), determined by its ability to inhibit the mouse IL-4 dependent proliferation of mouse HT-2 cells. TGF-b2 has growth inhibitory action and regulates the proliferation and differentiation of normal and transformed cells. Differential expression of TGF isoforms is observed in certain disease states. Human PC-3 prostatic carcinoma cell line and human glioblastoma cell lines predominantly secrete the TGF-b2 isoform over the other isoforms.
• Endotoxin Level: ~0.1ng/ug
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for TGFB2 active protein

TGF-B is a multifunctional polypeptide growth factor. While specific receptors for this protein have been found on almost all mammalian cell types, the effect of the molecule varies depending on the cell type and growth conditions. Generally, TGF-B is stimulatory for cells of mesenchymal origin and inhibitory for cells of epithelial or neuroectodermal origin. It is a family of regulatory proteins consisting of a number of proteins distantly related. TGF-B1 has been found in the highest concentration in human platelets and mammalian bone, but is produced by many cell types in smaller amounts. TGF-B2 has been found in the highest concentration in porcine platelets and mammalian bone, but again is also produced by many types of cells. The heterodimer, TGF-B1.2, has so far been found only in small amounts in porcine platelets. TGF-B3 has been detected in human, porcine, and avian sources, mainly in cells of mesenchymal origin, suggesting a different role for this protein than for TGF-B1 or TGF-B2. TGF-B4 has been detected so far only in chick embryo chondrocytes, and its distribution in other types of cells has not yet been characterized. TGF-B5 has been detected only in Xenopus embryos. TGF-B1, TGF-B2, and TGF-B1.2 appear to be largely equivalent in biological activity, although there appear to be differences in binding to certain types of receptors, and there are a few reports of differential responses to TGF-B1 and TGF-B2.

Product Categories/Family for TGFB2 active protein

Growth Factors; Cytokines; Growth Factors-TGF

NCBI and Uniprot Product Information

• NCBI GI #: 557563
• NCBI GeneID: 7042
• UniProt Accession #: P61812; P08112; Q15579; Q15581; Q4VAV9; B4DKC5; Q59EG9
• Molecular Weight: 25.0kD, 112 amino acid residues
• NCBI Official Full Name: transforming growth factor beta 2
• NCBI Official Synonym Full Names: transforming growth factor, beta 2
• NCBI Official Symbol: TGFB2
• NCBI Official Synonym Symbols: MGC116892; TGF-beta2
• NCBI Protein Information: transforming growth factor beta-2; G-TSF; cetermin; polyergin; TGF-beta-2; OTTHUMP00000037528; OTTHUMP00000037529; BSC-1 cell growth inhibitor; glioblastoma-derived T-cell suppressor factor
• UniProt Protein Name: Transforming growth factor beta-2
• Protein Family: Transforming growth factor
• UniProt Gene Name: TGFB2
• UniProt Entry Name: TGFB2_HUMAN

NCBI Description

This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types by transducing their signal through combinations of transmembrane type I and type II receptors (TGFBR1 and TGFBR2) and their downstream effectors, the SMAD proteins. Disruption of the TGFB/SMAD pathway has been implicated in a variety of human cancers. The encoded protein is secreted and has suppressive effects of interleukin-2 dependent T-cell growth. Translocation t(1;7)(q41;p21) between this gene and HDAC9 is associated with Peters' anomaly, a congenital defect of the anterior chamber of the eye. The knockout mice lacking this gene show perinatal mortality and a wide range of developmental, including cardiac, defects. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Uniprot Description

Function: TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.

Subunit structure: Homodimer; disulfide-linked. Interacts with ASPN. Ref.11

Subcellular location: Secreted.

Involvement in disease: Note=A chromosomal aberration involving TGFB2 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with HDAC9.

Sequence similarities: Belongs to the TGF-beta family.