Thyroid Stimulating Hormone alpha, beta Active Protein | TSHB active protein

Thyroid Stimulating Hormone alpha, beta, Recombinant, Human (TSHa, TSHb)

Cat. Num. AAA637281

• Gene Names: TSHB; TSH-B; TSH-BETA
• Purity: >90%, as determined by SDS-PAGE andvisualized by silver stain and blue stain.
• Synonyms: Thyroid Stimulating Hormone alpha; beta; Recombinant; Human (TSHa; TSHb); TSHB active protein

• Purity/Purification: >90%, as determined by SDS-PAGE andvisualized by silver stain and blue stain.
• Form/Format: Supplied as a lyophilized powder in PBS,pH 7.4, containing 0.05mg/ug BSA.Reconstitute with 100ul sterile PBS
• Concentration: ~0.1mg/ml (after reconstitution) (varies by lot)

• Activity: Measured by its ability to induce cAMPaccumulation in human TSH receptortransfected HEK293 cells. ED500.25-1.5ng/ml
• Endotoxin: <1EU/ug (LAL)
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for TSHB active protein

Thyroid-stimulating hormone (TSH orthyrotropin), produced in humanpituitary glands, has a molecular mass of25kD. An Important indicator of thyroid function, TSH is used to monitor thyroid associated diseases. Thyroid-stimulating hormone is a hormone synthesized and secreted by thyrotrope cells in the anterior pituitary gland which regulates the endocrine function of the thyroidgland. TSH stimulates the thyroid gland to secrete the hormones thyroxine (T4)and triiodothyronine (T3). TSH production is controlled by a Thyrotropin Releasing Hormone (TRH), which ismanufactured in the hypothalamus andtransported to the anterior pituitarygland, where it increases TSH productionand release. Somatostatin is also produced by the hypothalamus, and hasan opposite effect on the pituitary production of TSH, decreasing or inhibiting its release. The level of Thyroid hormones (T3 and T4) in the blood have an additional effect on the pituitary release of TSH. When the levels of T3 and T4 are low, the production of TSH is increased, and conversely, when levels of T3 and T4 are high, then TSH production is decreased. This effect creates a regulatory negative feedback loop. TSH is a glycoprotein and consists of two subunits, the alpha and the beta subunit. The alpha subunit is identical to that of human chorionicgonadotropin (HCG), luteinizing hormone(LH), follicle-stimulating hormone (FSH).The beta subunit is unique to TSH, and therefore determines its function. Structure and charge of the three N-linked carbohydrate chains influence activity; the most complex forms have lower activity but longer half life. Bovine and porcine TSH bind human TSH receptors (TSHR) with high affinity. he hypothalamic peptide TRH stimulates production and secretion of TSH by thyrotrophs (basophile cells) in the anterior pituitary gland. TSH travels tothyroid TSHR to stimulate production of thyroxine (T4). In the tissues, T4 is converted to the active form of thyroidhormone, triiodothyronine (T3), which completes a feedback loop by inhibiting TSH production. Studies in mouse identify bone marrow as a secondary site of TSH production. In bone, TSH signaling through TSHR on osteoblast and osteoclast precursors negatively regulates skeletal remodeling.

Description: DNA sequences encoding human TSH-a(Ala25-Ser116, UniProt Accession:P01215) and human TSH-b(Phe21-Val138, UniProt Accession:P01222) were co-expressed in Chinese Hamster Ovary cells.

Product Categories/Family for TSHB active protein

Molecular Biology; MB-Hormones; Steroids

NCBI and Uniprot Product Information

• NCBI GI #: 115430099
• NCBI GeneID: 7252
• NCBI Accession #: NP_000540.2
• NCBI GenBank Nucleotide #: NM_000549.4
• UniProt Accession #: P01222; Q16163; B1AKP0
• Molecular Weight: Predicted: 10.2 kD (TSH alpha) and 13.5kD (TSH beta)Obtained by SDS-PAGE: 15-29 kD underreducing conditions
• NCBI Official Full Name: thyrotropin subunit beta isoform 1
• NCBI Official Synonym Full Names: thyroid stimulating hormone, beta
• NCBI Official Symbol: TSHB
• NCBI Official Synonym Symbols: TSH-B; TSH-BETA
• NCBI Protein Information: thyrotropin subunit beta; thyrotropin beta chain
• UniProt Protein Name: Thyrotropin subunit beta
• Protein Family: Thyrotropin
• UniProt Gene Name: TSHB
• UniProt Synonym Gene Names: TSH-B; TSH-beta
• UniProt Entry Name: TSHB_HUMAN

NCBI Description

The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. Thyroid stimulating hormone functions in the control of thyroid structure and metabolism. The protein encoded by this gene is the beta subunit of thyroid stimulating hormone. Mutations in this gene are associated with congenital central and secondary hypothyroidism and Hashimoto's thyroiditis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]

Uniprot Description

TSHB: Indispensable for the control of thyroid structure and metabolism. Belongs to the glycoprotein hormones subunit beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: extracellular region

Molecular Function: hormone activity

Biological Process: G-protein coupled receptor protein signaling pathway; anatomical structure morphogenesis; cellular protein metabolic process; cell-cell signaling; response to estrogen stimulus; peptide hormone processing; response to calcium ion; response to vitamin A

Disease: Hypothyroidism, Congenital, Nongoitrous, 4