RSPO-1 Active Protein | RSPO1 active protein

RSPO-1, Recombinant, Human (R-Spondin 1, Roof Plate-specific Spondin1) (BSA & Azide Free)

Cat. Num. AAA637191

• Gene Names: RSPO1; RSPO; CRISTIN3; RP11-566C13.1
• Purity: Purified; 90% (SDS­PAGE) under reducing conditions and visualized by silver stain. Endotoxin: 1EU/ug (LAL)
• Synonyms: RSPO-1; Recombinant; Human (R-Spondin 1; Roof Plate-specific Spondin1) (BSA & Azide Free); RSPO1 active protein

• Purity/Purification: Purified; 90% (SDS­PAGE) under reducing conditions and visualized by silver stain. Endotoxin: 1EU/ug (LAL)
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile PBS to 0.1mg/ml.

• Application Notes: SDS-PAGE: 39kD, reducing conditions.
• Biological Activity: Measured by its' ability to induce activation of b-catenin response in a Topflash Luciferase assay using HEK293T. The ED50 for this effect is typically 1-4ng/ml in the presence of 5ng/ml rmWnt-3a.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute with sterile PBS. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for RSPO1 active protein

R­Spondin 1 (RSPO1, Roof plate­specific Spondin 1), also known as cysteine­rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27kD secreted protein that shares ~40% amino acid (aa) identity with three other R­Spondin family members. All R­Spondins regulate Wnt/b­catenin signaling, but have distinct expression patterns. Like other R­Spondins, R­Spondin 1 contains two adjacent cysteine­rich furin­like domains (aa34­135) with one potential N­glycosylation site, followed by a thrombospondin (TSP­1) motif (aa147­207) and a region rich in basic residues (aa211­263). Only the furin­like domains are needed for b­catenin stabilization. A putative nuclear localization signal at the C­terminus may allow some expression in the nucleus. Potential isoforms of 200 and 236aa have an alternate, shorter N­terminus or are missing aa146­208, respectively. Over aa21­263, human R­Spondin 1 shares 89%, 87%, 92%, 91%, 91% and 89% aa identity with mouse, rat, equine, canine, caprine and bovine RSPO­1, respectively. R­Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development. In humans, rare disruptions of the R­Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R­Spondin 1 in gender­specific differentiation. Disruption is also associated with palmoplantar keratosis. Postnatally, R­Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate. Injection of recombinant R­Spondin 1 in mice causes activation of b­catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis. R­Spondin 1 regulates Wnt/b­catenin by competing with the Wnt antagonist DKK­1 for binding to the Wnt co­receptors, Kremen and LRP­6, reducing their DKK­1­mediated internalization. Reports differ on whether R­Spondin 1 binds LRP­6 directl

Product Categories/Family for RSPO1 active protein

Molecular Biology; MB-Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 339276103
• NCBI GeneID: 284654
• NCBI Accession #: NP_001229839.1
• NCBI GenBank Nucleotide #: NM_001242910.1
• UniProt Accession #: Q2MKA7; Q0H8S6; Q14C72; Q5T0F2; Q8N7L5; A2A420
• Molecular Weight: ~39kD
• NCBI Official Full Name: R-spondin-1 isoform 3
• NCBI Official Synonym Full Names: R-spondin 1
• NCBI Official Symbol: RSPO1
• NCBI Official Synonym Symbols: RSPO; CRISTIN3; RP11-566C13.1
• NCBI Protein Information: R-spondin-1; R-spondin homolog; roof plate-specific spondin-1
• UniProt Protein Name: R-spondin-1
• Protein Family: R-spondin
• UniProt Gene Name: RSPO1
• UniProt Synonym Gene Names: hRspo1
• UniProt Entry Name: RSPO1_HUMAN

NCBI Description

This gene is a member of the R-spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. [provided by RefSeq, Jul 2008]

Uniprot Description

R-spondin-1: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC). This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Cell development/differentiation; Cell adhesion

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: extracellular space; extracellular region; nucleus

Molecular Function: heparin binding; protein binding; G-protein-coupled receptor binding; receptor binding

Biological Process: regulation of gene expression; regulation of receptor internalization; Wnt receptor signaling pathway through beta-catenin; positive regulation of protein amino acid phosphorylation; male meiosis; positive regulation of Wnt receptor signaling pathway

Disease: Palmoplantar Hyperkeratosis With Squamous Cell Carcinoma Of Skin And 46,xx Sex Reversal