PLTP active protein

PLTP, Recombinant, Mouse (Phospholipid Transfer Protein)

Cat. Num. AAA635851

• Gene Names: PLTP; BPIFE; HDLCQ9
• Purity: Purified; Purified 95% (SDS-PAGE). Endotoxin: 1.0 EU/1ug (LAL).
• Synonyms: PLTP; Recombinant; Mouse (Phospholipid Transfer Protein); PLTP active protein

• Specificity: Recognizes mouse PLTP.
• Purity/Purification: Purified; Purified 95% (SDS-PAGE). Endotoxin: 1.0 EU/1ug (LAL).
• Form/Format: Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, 50ug of BSA per ug of protein. Reconstitute with 40-50% glycerol, PBS.

• Immunogen: NS0, recombinant mouse PLTP, Glu 18-Ala 493; AAH03782.
• Activity: Measured by its ability to inhibit LPS-induced TNF-alpha secretion by Raw264.7 cells.
• Preparation and Storage: 12 months from date of receipt, 20 to 70 degree C as supplied.; 1 month, 2 to 8 degree C under sterile conditions after reconstitution.; 3 months, 20 to 70 degree C under sterile conditions after reconstitution.; Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Related Product Information for PLTP active protein

Mouse PLTP Phospholipid transfer protein (PLTP; also lipid transfer protein II) is an 80kD, secreted glycoprotein, that belongs to the BPI/LBP/Plunc superfamily, BPI/LBP family of molecules (1). It is synthesized as a precursor with a 17aa signal sequence and a 476aa mature chain (SwissProt # P55065). The mature chain contains two BPI/LBP/CETP domains (aa21-239 and 263-463) and seven potential sites for N-linked glycosylation. Mature mouse PLTP shares 83% aa sequence identity with mature human PLTP (3). PLTP is expressed by a wide variety of tissues and is secreted by macrophages. Northern blot analysis shows the order of PLTP mRNA expression as follows: lung > adipose tissue, placenta, testis > brain > muscle, heart, liver (2). PLTP is a multifunctional, extracellular lipid transfer protein that plays a major role in lipoprotein metabolism and atherosclerosis (4-6). In addition to phospholipids, PLTP transfers several amphipatic compounds, including unesterified cholesterol, lipopolysaccharides, diacylglycerides, and alpha-tocopherol, the main isomer of vitamin E (4). According to several studies, PLTP not only affects plasma lipid and lipoprotein profiles, but also modulates cell physiology (4, 7-11). In mice, systemic PLTP deficiency proves to be atheroprotective, whereas PLTP overexpression is proatherogenic (4, 6). However, macrophage PLTP is shown to be atheroprotective (4, 6).

Product Categories/Family for PLTP active protein

Molecular Biology; MB-Serum Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 339275810
• NCBI GeneID: 5360
• NCBI Accession #: NP_001229850.1
• NCBI GenBank Nucleotide #: NM_001242921.1
• UniProt Accession #: P55058; Q8WTT1; Q9BR07; Q9BSH8; A8K006; B4DRB4; E1P5N8; E7EV16
• Molecular Weight: Based on N-terminal amino acid sequencing, the recombinant mouse PLTP starts at Glu 18 and has a calculated molecular mass of approximately 53.5kD. The recombinant mouse PLTP migrates as an approximately 70-85kD protein in SDS-PAGE under reducing conditio
• NCBI Official Full Name: phospholipid transfer protein isoform d
• NCBI Official Synonym Full Names: phospholipid transfer protein
• NCBI Official Symbol: PLTP
• NCBI Official Synonym Symbols: BPIFE; HDLCQ9
• NCBI Protein Information: phospholipid transfer protein; lipid transfer protein II; BPI fold containing family E
• UniProt Protein Name: Phospholipid transfer protein
• Protein Family: Phospholipid transfer protein
• UniProt Gene Name: PLTP
• UniProt Entry Name: PLTP_HUMAN

NCBI Description

The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL). In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PLTP: Converts HDL into larger and smaller particles. May play a key role in extracellular phospholipid transport and modulation of hdl particles. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Lipid-binding; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: extracellular space; extracellular region

Molecular Function: lipid binding

Biological Process: sperm motility; vitamin E biosynthetic process; lipid metabolic process; lipid transport