MGMT, active Active Protein | MGMT active protein

MGMT, Recombinant, Human, active (O6-Methylguanine DNA Methyltransferase)

Cat. Num. AAA635844

• Purity: Highly Purified; 0.9
• Synonyms: MGMT; active; Recombinant; Human; active (O6-Methylguanine DNA Methyltransferase); MGMT active protein

• Purity/Purification: Highly Purified; 0.9
• Form/Format: Supplied as a liquid in 20mM Tris, pH 7.5, 1mM DTT, 1mM EDTA, 200mM sodium chloride and 50% glycerol.

• Specific Activity: Minimum 10,000 Units/mg protein. One unit is defined as the amount of protein that cleaves 50% of 0.5 pmole of dsDNA oligomer substrate (O6-methylguanine) in 10 min at 37 degree C.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for MGMT active protein

Many tumor promoting/causing agents (BCNU, CCNU, DTIC, procarbazine, temozolomide, etc) generate lethal cross-links at the O6-alkylguanine position in DNA. MGMT (O6-Methylguanine DNA Methyltransferase) removes alkyl adducts from the O6-position of guanine in DNA (prior to cross-link formation) to its cysteine residue, thereby irreversibly inactivating MGMT. Inactivated MGMT is rapidly degraded. Therefore, MGMT levels serves as an indicator of DNA damage.

Product Categories/Family for MGMT active protein

Molecular Biology; MB-Enzymes; Methyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 127069
• NCBI GeneID: 4255
• UniProt Accession #: P16455; Q5VY78
• Molecular Weight: 21,646 Da
• NCBI Official Full Name: Methylated-DNA--protein-cysteine methyltransferase
• NCBI Official Synonym Full Names: O-6-methylguanine-DNA methyltransferase
• NCBI Official Symbol: MGMT
• NCBI Protein Information: methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase
• UniProt Protein Name: Methylated-DNA--protein-cysteine methyltransferase
• Protein Family: Probable methylated-DNA--protein-cysteine methyltransferase
• UniProt Gene Name: MGMT
• UniProt Synonym Gene Names: MGMT
• UniProt Entry Name: MGMT_HUMAN

Uniprot Description

MGMT: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Belongs to the MGMT family.

Protein type: DNA repair, damage; EC 2.1.1.63; Apoptosis; Methyltransferase; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: nucleoplasm; membrane; nucleus

Molecular Function: methyltransferase activity; methylated-DNA-[protein]-cysteine S-methyltransferase activity; DNA binding; damaged DNA binding; calcium ion binding; DNA-methyltransferase activity

Biological Process: response to drug; DNA dealkylation; positive regulation of DNA repair; response to ethanol; response to folic acid; response to toxin; DNA methylation; DNA repair; regulation of caspase activity; DNA ligation