Membrane Frizzled-Related Protein Active Protein | MFRP active protein

Membrane Frizzled-Related Protein, Recombinant, Human (Membrane-type Frizzled-Related Protein, MFRP)

Cat. Num. AAA635899

• Gene Names: MFRP; RD6; NNO2; MCOP5
• Purity: Highly Purified; 90%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: Membrane Frizzled-Related Protein; Recombinant; Human (Membrane-type Frizzled-Related Protein; MFRP); MFRP active protein

• Purity/Purification: Highly Purified; 90%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in PBS.

• Activity: Measured by its ability to inhibit the activity of rhBMP-1 on the cleavage of a fluorogenic peptide substrate, Mca-Y-V-A-D-A-P-K(Dnp)-OH. Cleavage of ES007 can be measured using excitation and emission wavelengths of 320nm and 405nm, respectively. 30ug/ml of rhMFRP reduces rhBMP-1 activity by 30-60% when measured with 1ug of BMP-1 and 10uM ES007 in 100ul of 10 mM HEPES, 0.01% Brij 35, pH 7.5 at RT.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for MFRP active protein

MFRP (membrane-type frizzled-related protein) is a 65kD, type II transmembrane protein related to both Tolloid proteases and frizzled-domain containing Wnt pathway proteins (1-4). Human MFRP is 579 amino acids (aa) in length (3, 5, 6). It contains a 69aa cytoplasmic region, a 21aa transmembrane segment, and a 489aa extracellular domain (ECD). The ECD is characterized by the presence of two LDLR class A repeats, two CUB domains, and a C-terminal cysteine-rich/frizzled domain. The mRNA for MFRP is highly unusual in that it is dicistronic; that is, it contains two independent ORFs, one for MFRP and one for a functionally- related protein termed CTRP5/C1qTNF5 (4, 7). CTRP5 is a secreted, 25kD short-chain collagen that contains a C1q-type domain (6, 8). In prokaryotes, polycistronic transcripts exist that contain functionally-interactive molecules. This would also appear to be the case for MFRP and CTRP5. CTRP5 is suggested to bind to membrane MFRP via the C1q and CUB domains, respectively. This is posited to generate a receptor-coreceptor complex that binds select Wnts such as Wnt-1 and/or Wnt-10b (4, 6, 7). MFRP has multiple documented mutations. In human, these are associated with hyperopia (severe farsightedness). The mutations result in premature truncations (3, 9). MFRP is expressed in retinal pigment epithelium, ciliary epithelium, and keratinocytes (4, 7, 10, 11). Human MFRP ECD is 70% aa identical to mouse ECD.

Product Categories/Family for MFRP active protein

Molecular Biology; MB-Wnt Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 13899255
• NCBI GeneID: 83552
• NCBI Accession #: NP_113621.1
• NCBI GenBank Nucleotide #: NM_031433.3
• UniProt Accession #: Q9BY79; Q335M3; Q96DQ9; B0YJ36; B0YJ37
• Molecular Weight: Based on N-terminal amino acid sequencing, the recombinant human MFRP begins with the histidine tag. It has a predicted molecular mass of approximately 52.7kDa As a result of glycosylation, the recombinant monomer migrates as an approximately 90-105kD pro
• NCBI Official Full Name: membrane frizzled-related protein
• NCBI Official Synonym Full Names: membrane frizzled-related protein
• NCBI Official Symbol: MFRP
• NCBI Official Synonym Symbols: RD6; NNO2; MCOP5
• NCBI Protein Information: membrane frizzled-related protein; membrane-type frizzled-related protein
• UniProt Protein Name: Membrane frizzled-related protein
• Protein Family: Membrane frizzled-related protein
• UniProt Gene Name: MFRP
• UniProt Entry Name: MFRP_HUMAN

NCBI Description

This gene encodes a member of the frizzled-related protein family. The encoded protein plays an important role in eye development and mutations in this gene have been associated with nanophthalmos, posterior microphthalmia, retinitis pigmentosa, foveoschisis, and optic disc drusen. The protein is encoded by a bicistronic transcript which also encodes C1q and tumor necrosis factor related protein 5 (C1QTNF5). [provided by RefSeq, Jun 2013]

Uniprot Description

MFRP: May play a role in eye development. Defects in MFRP are the cause of nanophthalmos 2 (NNO2). NNO2 is a rare autosomal recessive disorder of eye development characterized by extreme hyperopia and small functional eyes. Defects in MFRP are the cause of microphthalmia isolated type 5 (MCOP5). Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. MCOP5 is characterized by posterior microphthalmia, retinitis pigmentosa, foveoschisis and optic disc drusen.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: apical plasma membrane; integral to membrane

Biological Process: embryonic development

Disease: Microphthalmia, Isolated 5; Nanophthalmos 2