Macrophage Inflammatory Protein 3 alpha Active Protein | CCL20 active protein

Macrophage Inflammatory Protein 3 alpha, Recombinant, Human (MIP-3a, Exodus, LARC)

Cat. Num. AAA650652

• Gene Names: CCL20; CKb4; LARC; ST38; MIP3A; MIP-3a; SCYA20
• Purity: Highly Purified; ~98% (SDS-PAGE, HPLC)
• Synonyms: Macrophage Inflammatory Protein 3 alpha; Recombinant; Human (MIP-3a; Exodus; LARC); CCL20 active protein

• Purity/Purification: Highly Purified; ~98% (SDS-PAGE, HPLC)
• Form/Format: Supplied as a lyophilized powder. BSA free. Reconstitute with sterile dH2O to 0.1-1mg/ml.

• Biological Activity: The biological activity of recombinant human MIP-3a is determined by measuring concentration dependent chemotaxis with human T cells. A concentration of 10-50ng/ml produces significant chemotaxis. The optimal concentration should be determined for each specific application. MIP-3a is a b chemokine (C-C chemokine).
• Endotoxin Level: 0.1ng/ug
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for CCL20 active protein

Growth Factors; Cytokines; Growth Factors-MIP

NCBI and Uniprot Product Information

• NCBI GI #: 1790925
• NCBI GeneID: 6364
• UniProt Accession #: P78556; Q53S51; Q99664
• Molecular Weight: 10,762 Da
• NCBI Official Full Name: macrophage inflammatory protein 3 alpha
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 20
• NCBI Official Symbol: CCL20
• NCBI Official Synonym Symbols: CKb4; LARC; ST38; MIP3A; MIP-3a; SCYA20
• NCBI Protein Information: C-C motif chemokine 20; exodus-1; MIP-3-alpha; CC chemokine LARC; OTTHUMP00000164245; OTTHUMP00000204113; beta chemokine exodus-1; beta-chemokine exodus-1; small-inducible cytokine A20; macrophage inflammatory protein 3 alpha; liver and activation-regulated chemokine; small inducible cytokine subfamily A (Cys-Cys), member 20
• UniProt Protein Name: C-C motif chemokine 20
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL20
• UniProt Synonym Gene Names: LARC; MIP3A; SCYA20
• UniProt Entry Name: CCL20_HUMAN

NCBI Description

This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

Function: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. Ref.8

Subunit structure: Binds to and activate CCR6.

Subcellular location: Secreted.

Tissue specificity: Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon.

Induction: By bacterial lipopolysaccharides (LPS), TNF and IFNG/IFN-gamma. Induced by phorbol myristate acetate (PMA) in U937 cell line and bowes melanoma. Repressed by IL10/interleukin-10.

Post-translational modification: C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.

Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.