Interleukin 9, Rat Active Protein | IL9 active protein

Interleukin 9, Recombinant, Rat (IL-9) (BSA Free)

Cat. Num. AAA650944

• Gene Names: IL9; P40; HP40; IL-9
• Purity: Highly Purified; 90%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: Interleukin 9; Rat; Recombinant; Rat (IL-9) (BSA Free); IL9 active protein

• Purity/Purification: Highly Purified; 90%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in 30% CH3CN, 0.1% TFA.

• Activity: The biological activity of recombinant rIL-9 was measured in a cell proliferation assay using a factor-dependent mouse cell line, TS1 (Uyttenhove, C. et. al., 1988, Proc. Natl. Acad. Sci. 85:6934-6938). The ED 50 for this effect is typically 3-15 ng/mL.
• Endotoxin Level: < 1.0 EU per 1 g of the protein as determined by the LAL method.
• Reconstitution: It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
• Preparation and Storage: Lyophilized samples are stable for up to twelve months at -20 degree C. Upon reconstitution, this protein, in the presence of a carrier protein, can be stored under sterile conditions at 2 degree -8 degree C for one month or at -20 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Related Product Information for IL9 active protein

Rat interleukin-9 (IL-9; also T-cell growth factor P40 and MEA) is a 20-30 kD secreted glycoprotein that belongs to the IL-7/IL-9 family of hematopoietic cytokines. It is best considered an atypical Th2-type cytokine. 1-4 Rat IL-9 was initially isolated from the 4437A rat thymoma cell line. 5 The IL-9 precursor is 144 amino acids (aa) in length and contains an 18 aa signal sequence plus a 126 aa mature segment. 5 An extended, 219 aa alternate precursor form is reported in GenBank (Accession # XP_001068265). It shows a 113 aa substitution for the first 38 aa of the standard form. Its significance is unknown. Mature rat IL-9 is 75%, 74% and 58% aa identical to cotton rat, mouse and human IL-9, respectively. 6-9 There is variability in cross-species reactivity. Canine and mouse IL-9 are purportedly active on human cells, 9-11 while human IL-9 is inactive on mouse cells. 9 Cells reported to express IL-9 include activated CD4+ T cells and eosinophils. 12, 13

Product Categories/Family for IL9 active protein

Growth Factors; Cytokines; Growth Factors-Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 10834980
• NCBI GeneID: 3578
• NCBI Accession #: NP_000581.1
• NCBI GenBank Nucleotide #: NM_000590.1
• UniProt Accession #: P15248; Q6NZ74; Q6NZ75
• Molecular Weight: The mature recombinant rat IL-9, generated by proteolytic removal of the signal peptide region, is a monomer protein. Based on N-terminal amino acid sequencing, the recombinant rat IL-9 starts at Gln 19. It has a calculated molecular mass of 14.1 kD. As a
• NCBI Official Full Name: interleukin-9
• NCBI Official Synonym Full Names: interleukin 9
• NCBI Official Symbol: IL9
• NCBI Official Synonym Symbols: P40; HP40; IL-9
• NCBI Protein Information: interleukin-9; cytokine P40; p40 cytokine; OTTHUMP00000159423; T-cell growth factor p40; p40 T-cell and mast cell growth factor; homolog of mouse T cell and mast cell growth factor 40
• UniProt Protein Name: Interleukin-9
• Protein Family: Interleukin
• UniProt Gene Name: IL9
• UniProt Entry Name: IL9_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. [provided by RefSeq]

Uniprot Description

Function: Supports IL-2 independent and IL-4 independent growth of helper T-cells.

Subcellular location: Secreted.

Sequence similarities: Belongs to the IL-7/IL-9 family.