Interleukin 22R Active Protein | IL22RA1 active protein

Interleukin 22R, Recombinant, Human (IL-22R, CRF2-9, IL-TIF R1, IL-10 Related T Cell-derived Inducible Factor)

Cat. Num. AAA650939

• Gene Names: IL22RA1; IL22R; CRF2-9; IL22R1
• Purity: >.95%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: Interleukin 22R; Recombinant; Human (IL-22R; CRF2-9; IL-TIF R1; IL-10 Related T Cell-derived Inducible Factor); IL22RA1 active protein

• Purity/Purification: >.95%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder from PBS, 7.4. Reconstitute with 500 ul sterile PBS..

• Source: Recombinant protein corresponding to Pro 18-Thr228 with C-terminal 6-His-Tag from human IL-22R, expressed in NS0 cells (NP_067081)
• SDS-PAGE: 34-38kD under reducing conditions.
• Endotoxin: <0.1 EU/ug (LAL)
• Activity: Measured by its ability to inhibit IL-22-induced IL-10 secretion by COLO 205 human colorectal adenocarcinoma cells. The ED50 for this effect is typically 1-4 ug/ml in the presence of 1ng/ml of rhIL-22.
• Preparation and Storage: Lyophilized powder may be stored at -20°C. Stable for 6 months afte receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for IL22RA1 active protein

IL-22 receptor, also known as IL-22R 1 and CRF2-9, is an approximately 65 kD transmembrane glycoprotein in the type II cytokine receptor family (CRF). IL-22R contains a 211 aa extracellular domain (ECD) with two fibronectin type III repeats, and a 323 aa cytoplasmic domain. IL-22R associates with either IL-10R or IL-20R to form receptor complexes with distinct ligand selectivities. IL-10R is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20R is a shared subunit of the IL-19, -20, -22R and -24 receptors. 1 IL-22R/IL-10 R is an IL-22 responsive receptor 2, 3, and IL-22R/IL-20R is an IL-20 or IL-24 responsive receptor. 4, 5 IL-22R contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL-10R or IL-20R and the respective ligands is required for signal transduction. 2, 6 IL-22BP functions as a competitive antagonist by binding IL-22 and preventing its association with IL-22R. 7, 9 Even though it is a receptor for interleukins, IL-22R is not expressed on hematopoietic cells. 6, 10, 11 Instead, IL-22R expression is restricted to epithelial and stromal cells. 6, 10-13 IL-22R signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases. 3, 11, 13, 14 IL-22R signaling also promotes downregulation of proteins involved in keratinocyte differentiation. 3, 14 Within the ECD, human IL-22R shares 78%, 76%, and 83% aa sequence identity with mouse, rat, and canine IL-22R, respectively. It shares 22%-25% aa sequence identity with the ECDs of other class II receptors IL-10R, IL-20R, and IL-28R.

Product Categories/Family for IL22RA1 active protein

Growth Factors; Cytokines; Growth Factors-Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 31317239
• NCBI GeneID: 58985
• NCBI Accession #: NP_067081.2
• NCBI GenBank Nucleotide #: NM_021258.3
• UniProt Accession #: Q8N6P7; Q9HB22; A8K839; B2R9Y9
• Molecular Weight: 24.9kD
• NCBI Official Full Name: interleukin-22 receptor subunit alpha-1
• NCBI Official Synonym Full Names: interleukin 22 receptor, alpha 1
• NCBI Official Symbol: IL22RA1
• NCBI Official Synonym Symbols: IL22R; CRF2-9; IL22R1
• NCBI Protein Information: interleukin-22 receptor subunit alpha-1; IL-22RA1; zcytoR11; IL-22R-alpha-1; IL-22 receptor subunit alpha-1; cytokine receptor class-II member 9; cytokine receptor family 2 member 9
• UniProt Protein Name: Interleukin-22 receptor subunit alpha-1
• Protein Family: Interleukin-22 receptor
• UniProt Gene Name: IL22RA1
• UniProt Synonym Gene Names: IL22R; IL-22 receptor subunit alpha-1; IL-22R-alpha-1; IL-22RA1; CRF2-9
• UniProt Entry Name: I22R1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the class II cytokine receptor family, and has been shown to be a receptor for interleukin 22 (IL22). IL22 receptor is a protein complex that consists of this protein and interleukin 10 receptor, beta (IL10BR/CRFB4), a subunit also shared by the receptor complex for interleukin 10 (IL10). This gene and interleukin 28 receptor, alpha (IL28RA) form a cytokine receptor gene cluster in the chromosomal region 1p36. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Component of the receptor for IL20, IL22 and IL24. Component of IL22 receptor formed by IL22RA1 and IL10RB enabling IL22 signaling via JAK/STAT pathways. IL22 also induces activation of MAPK1/MAPK3 and Akt kinases pathways. Component of one of the receptor for IL20 and IL24 formed by IL22RA1 and IL20RB also signaling through STATs activation. Mediates IL24 antiangiogenic activity as well as IL24 inhibitory effect on endothelial cell tube formation and differentiation. Ref.6 Ref.7 Ref.9 Ref.10 Ref.11 Ref.14

Subunit structure: Heterodimer with IL10RB and with IL20RB. IL22 binding to heterodimer is greater than binding to IL22RA1 alone. Ref.6 Ref.13

Subcellular location: Membrane; Single-pass type I membrane protein.

Tissue specificity: Expressed in colon, liver, lung, pancreas and kidney. No expression in immune cells such as monocytes, T-cells, and NK-cells. Expressed in keratinocytes of normal skin as well as in psoriatic skin lesion. Detected in normal blood brain barrier endothelial cells as well as in multiple sclerosis lesions; Strongly expressed on central nervous system vessels within infiltrated multiple sclerosis lesions. Overexpressed in synovial fluid cells from rheumatoid arthritis and spondyloarthropathy patients. Ref.8 Ref.12 Ref.15 Ref.17 Ref.18

Induction: By IFNG/IFN-gamma in keratinocytes. Ref.12

Miscellaneous: Failure of medical and surgical therapy in Chronic rhinosinusitis with nasal polyps is associated with decreased expression of IL22RA1.

Sequence similarities: Belongs to the type II cytokine receptor family.Contains 2 fibronectin type-III domains.