Interleukin 10 Active Protein | IL10 active protein

Interleukin 10, Recombinant, Human (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

Cat. Num. AAA650143

• Gene Names: IL10; CSIF; TGIF; IL-10; IL10A; MGC126450; MGC126451
• Purity: Molecular Biology Grade; 98% by SDS-PAGE and HPLC
• Synonyms: Interleukin 10; Recombinant; Human (IL-10; Cytokine Synthesis Inhibitory Factor; CSIF); IL10 active protein

• Purity/Purification: Molecular Biology Grade; 98% by SDS-PAGE and HPLC
• Form/Format: Supplied as a lyophilized powder from 5mM sodium phosphate, pH 7.2, without additives. Reconstitute with 5mM Tris, pH 7.2 to a concentration of 0.1-1mg/ml.
• Sequence: MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N

• Biological Activity: Determined by the dose-dependent co-stimulation (with murine IL-4) of MC/9 cells. The ED50 was found to be 2ng/ml, corresponding to a specific activity of 5x10e5 units/mg.
• Country of Origin: USA
• Endotoxin Level: 0.1ng/ug
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for IL10 active protein

Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor

Product Categories/Family for IL10 active protein

Growth Factors; Cytokines; Growth Factors-Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 10835141
• NCBI GeneID: 3586
• NCBI Accession #: NP_000563.1
• NCBI GenBank Nucleotide #: NM_000572.2
• UniProt Accession #: P22301; Q6FGS9; Q6FGW4; Q6LBF4; Q71UZ1; Q9BXR7
• Molecular Weight: 20,517 Da
• NCBI Official Full Name: interleukin-10
• NCBI Official Synonym Full Names: interleukin 10
• NCBI Official Symbol: IL10
• NCBI Official Synonym Symbols: CSIF; TGIF; IL-10; IL10A; MGC126450; MGC126451
• NCBI Protein Information: interleukin-10; OTTHUMP00000034567; T-cell growth inhibitory factor; cytokine synthesis inhibitory factor
• UniProt Protein Name: Interleukin-10
• Protein Family: Interleukin
• UniProt Gene Name: IL10
• UniProt Entry Name: IL10_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Uniprot Description

Function: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

Subunit structure: Homodimer.

Subcellular location: Secreted.

Tissue specificity: Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

Sequence similarities: Belongs to the IL-10 family.