Insulin-Like Growth Factor I, Rat Active Protein | IGF1 active protein
Insulin-Like Growth Factor I, Recombinant, Rat (IGF1, Somatomedin C)
Gene Names
IGF1; IGFI; IGF-I; IGF1A
Purity
Purified97%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
Synonyms
Insulin-Like Growth Factor I; Rat; Recombinant; Rat (IGF1; Somatomedin C); IGF1 active protein
Purity/Purification
Purified
97%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
97%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitution: Reconstitute with sterile PBS to 0.1mg/ml.
Activity
Measured by its ability to induce proliferation of MCF-7, a human breast carcinoma cell line, under serum free conditions. The ED50 for this effect is typically 0.6-3ng/ml.
Endotoxin Level
1EU/ug (LAL)
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for IGF1 active protein
Insulin-like growth factor I is the dominant effector of growth hormone and is structurally homologous to proinsulin. Rat IGFI is synthesized as four precursor isoforms with alternate N- and C-terminal propeptides. These isoforms are differentially expressed by various tissues. The 7.6kD mature IGFI is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature rat IGFI shares 96% and 99% aa sequence identity with human and mouse IGFI, respectively, and exhibits cross-species activity. It shares 61% aa sequence identity with mature rat IGFII. Circulating IGFI is produced by hepatocytes, while local IGFI is produced by many other tissues in which it has paracrine effects. IGFI induces the proliferation, migration and differentiation of a wide variety of cell types during development and postnatally. IGFI regulates glucose and fatty acid metabolism, steroid hormone activity and cartilage and bone metabolism. It plays an important role in muscle regeneration and tumor progression. IGFI binds IGFI R, IGFII R, and the insulin receptor, although its effects are mediated primarily by IGFI R. Heterodimers of insulin receptor/IGFI R also exist and respond to IGFI but not to insulin stimulation. IGFI association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors.
Product Categories/Family for IGF1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
The methionyl form of recombinant rat IGF-I contains 78 amino acid residues and has a calculated molecular mass of 8.8kD.
NCBI Official Full Name
insulin-like growth factor I
NCBI Official Synonym Full Names
insulin-like growth factor 1 (somatomedin C)
NCBI Official Symbol
IGF1
NCBI Official Synonym Symbols
IGFI; IGF-I; IGF1A
NCBI Protein Information
insulin-like growth factor 1; MGF; IGF-IA; IGF-IB; somatomedin-C; OTTHUMP00000195080; OTTHUMP00000195081; OTTHUMP00000195082; OTTHUMP00000195083; OTTHUMP00000195084; mechano growth factor; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB
UniProt Protein Name
Insulin-like growth factor 1 (Somatomedin C), isoform CRA_c
Protein Family
UniProt Gene Name
IGF1
UniProt Entry Name
Q14620_HUMAN
NCBI Description
The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.
Uniprot Description
Subcellular location: Secreted
By similarity RuleBase RU000406.
Sequence similarities: Belongs to the insulin family. RuleBase RU000406
Research Articles on IGF1
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Product Notes
The IGF1 igf1 (Catalog #AAA650518) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Insulin-Like Growth Factor I, Rat, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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