IL-6 Active Protein | Il6 active protein

RECOMBINANT RAT INTERLEUKIN-6

Cat. Num. AAA232383

• Gene Names: Il6; ILg6; Ifnb2
• Applications: ELISA, Functional Assay, Western Blot
• Purity: >98% by SDS PAGE and HPLC analysis.
• Synonyms: IL-6; RECOMBINANT RAT INTERLEUKIN-6; Il6 active protein

• Purity/Purification: >98% by SDS PAGE and HPLC analysis.
• Form/Format: Purified recombinant protein expressed in E Coli - lyophilised.
• Concentration: Approximate Protein Concentration: 0.1 mg/ml after reconstitution (varies by lot)
• Sequence Length: 211

• Applicable Applications for Il6 active protein: ELISA (EIA), Functional Assays (FN), Western Blot (WB)
• Application Notes: ELISA: Recombinant rat IL-6 may be used as a standard for ELISA applications with either anti Rat IL-6: purified or anti Rat IL-6: biotin conjugate.; Western Blot: Recombinant Rat IL-6 may be used as the positive control for western blotting applications with either anti Rat IL-6: purified or anti Rat IL-6: biotin conjugate.; ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well; Functional Assays: Minimum Dilution: 0.01; Maximum Dilution: 10ng/ml; Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane
• Reconstitution: Reconstitute with 0.1 ml distilled water. For extended storage the addition of 0.1% BSA is recommended. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution.
• Buffer Solution: TRIS buffered saline; 0.1% SDS
• Activity: ED50 of < 0.01ng/ml when assayed by proliferation of IL-6 dependent murine 7TD1 cells. This corresponds to a specific activity of 1 x 108 units/mg.
• Target Species: Rat
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.; Shelf Life: 3 months from date of reconstitution.

Related Product Information for Il6 active protein

Recombinant rat IL-6 is a 21.7 kDa peptide containing 188 residues corresponding to full-length mature rat interleukin-6. IL-6 is a pleiotropic cytokine involved in the regulation of immune and inflammatory responses.

NCBI and Uniprot Product Information

• NCBI GI #: 7549769
• NCBI GeneID: 24498
• NCBI Accession #: NP_036721.1
• NCBI GenBank Nucleotide #: NM_012589.2
• UniProt Accession #: P20607
• Molecular Weight: Protein Molecular Weight: 21.7kD (188 Amino acids)
• NCBI Official Full Name: interleukin-6
• NCBI Official Synonym Full Names: interleukin 6
• NCBI Official Symbol: Il6
• NCBI Official Synonym Symbols: ILg6; Ifnb2
• NCBI Protein Information: interleukin-6; IL-6; Interleukin 6 (interferon, beta 2)
• UniProt Protein Name: Interleukin-6
• Protein Family: Interleukin
• UniProt Gene Name: Il6
• UniProt Synonym Gene Names: Il-6; IL-6
• UniProt Entry Name: IL6_RAT

NCBI Description

a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]

Uniprot Description

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation ().