IL-1alpha Active Protein | IL1A active protein

Recombinant Murine Interleukin?1 Alpha (rMuIL-1alpha)

Cat. Num. AAA546295

• Gene Names: IL1A; IL1; IL-1A; IL1F1; IL1-ALPHA
• Purity: > 97 % by SDS?PAGE and HPLC analyses.
• Synonyms: IL-1alpha; Recombinant Murine Interleukin?1 Alpha (rMuIL-1alpha); IL1A active protein

• Purity/Purification: > 97 % by SDS?PAGE and HPLC analyses.
• Form/Format: Lyophilized from a 0.2 ?m filtered solution in PBS, pH 7.4.
• Sequence: SAPYTYQSDL RYKLMKLVRQ KFVMNDSLNQ TIYQDVDKHY LSTTWLNDLQ QEVKFDMYAY SSGGDDSKYP VTLKISDSQL FVSAQGEDQP VLLKELPETP KLITGSETDL IFFWKSINSK NYFTSAAYPE LFIATKEQSR VHLARGLPSM TDFQIS
• Sequence Length: 271

• Source: Escherichia coli.
• Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 2 pg/ml, corresponding to a specific activity of > 5.0 × 10^8 IU/mg.
• Physical Appearance: Sterile Filtered White lyophilized (freeze/dried) powder.
• Endotoxin: Less than 1 EU/ug of rMuIL-1Alpha as determined by LAL method.
• Preparation and Storage: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

Related Product Information for IL1A active protein

Murine Interleukin-1 alpha: Interleukin-1 alpha (IL-1Alpha) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1Alpha and IL-1Beta binds to the same receptor and has similar if not identical biological properties. Among various species, the amino acid sequence of mature IL-1Alpha s conserved 60 % to 70 % and human IL1 has been found to be biologically active on murine cell lines

NCBI and Uniprot Product Information

• NCBI GI #: 27894330
• NCBI GeneID: 3552
• NCBI Accession #: NP_000566.3
• NCBI GenBank Nucleotide #: NM_000575.4
• Molecular Weight: Approximately 17.9 kDa, a single non?glycosylated polypeptide chain containing 156 amino acids.
• NCBI Official Full Name: interleukin-1 alpha
• NCBI Official Synonym Full Names: interleukin 1 alpha
• NCBI Official Symbol: IL1A
• NCBI Official Synonym Symbols: IL1; IL-1A; IL1F1; IL1-ALPHA
• NCBI Protein Information: interleukin-1 alpha
• UniProt Protein Name: Interleukin-1 alpha
• Protein Family: Interleukin
• UniProt Gene Name: IL1A
• UniProt Synonym Gene Names: IL1F1; IL-1 alpha
• UniProt Entry Name: IL1A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]

Uniprot Description

IL1A: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Belongs to the IL-1 family.

Protein type: Motility/polarity/chemotaxis; Cytokine

Chromosomal Location of Human Ortholog: 2q14

Cellular Component: cytosol; extracellular region; extracellular space

Molecular Function: copper ion binding; cytokine activity; interleukin-1 receptor binding; protein binding

Biological Process: apoptosis; cell proliferation; connective tissue replacement during inflammatory response; cytokine and chemokine mediated signaling pathway; fever; germ cell programmed cell death; immune response; inflammatory response; negative regulation of cell proliferation; positive regulation of angiogenesis; positive regulation of cytokine secretion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-2 biosynthetic process; positive regulation of JNK cascade; positive regulation of mitosis; positive regulation of protein secretion; positive regulation of transcription from RNA polymerase II promoter; response to copper ion