IL-10 Active Protein | Il10 active protein
RECOMBINANT RAT INTERLEUKIN-10
Gene Names
Il10; IL10X
Applications
ELISA, Functional Assay, Western Blot
Purity
>98% by SDS PAGE and HPLC analysis
Synonyms
IL-10; RECOMBINANT RAT INTERLEUKIN-10; Il10 active protein
Purity/Purification
>98% by SDS PAGE and HPLC analysis
Form/Format
Purified recombinant protein - lyophilised
Concentration
Approximate Protein Concentration: 1.0 mg/ml (varies by lot)
Sequence Length
178
Applicable Applications for Il10 active protein
ELISA (EIA), Functional Assays (FN), Western Blot (WB)
Application Notes
ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well;
Functional Assays: Minimum Dilution: 0.1; Maximum Dilution: 10.0ng/ml;
Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane
Functional Assays: Minimum Dilution: 0.1; Maximum Dilution: 10.0ng/ml;
Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane
Reconstitution
Reconstitute with 10ul water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For extended storage te addtion of 0.1% BSA is recommended,
Preparation
Preparation
Buffer Solution
Sodium Phosphate
Activity
Determined by the dose-dependent inhibition of antigen-specific T-cell proliferation is = 10ng/ml
Target Species
Rat
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 3 months from date of reconstitution.
Shelf Life: 3 months from date of reconstitution.
Related Product Information for Il10 active protein
IL-10 is a homodimeric protein, each 18.7kD subunit is 160 amino acid residues in length. IL-10 is a potent inhibitor of several cytokines including IFN gamma, IL-2 and TNF. Interleukin-10 (IL-10) is produced primarily by activated T lymphocytes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Protein Molecular Weight: 18.7kD (160 amino acid sequence)
NCBI Official Full Name
interleukin-10
NCBI Official Synonym Full Names
interleukin 10
NCBI Official Symbol
Il10
NCBI Official Synonym Symbols
IL10X
NCBI Protein Information
interleukin-10; CSIF; IL-10; cytokine synthesis inhibitory factor
UniProt Protein Name
Interleukin-10
Protein Family
UniProt Gene Name
Il10
UniProt Synonym Gene Names
Il-10; IL-10; CSIF
UniProt Entry Name
IL10_RAT
NCBI Description
factor involved in the inhibition of cytokine synthesis [RGD, Feb 2006]
Uniprot Description
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Research Articles on Il10
Similar Products
Product Notes
The Il10 il10 (Catalog #AAA232391) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IL-10 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Functional Assays (FN), Western Blot (WB). ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well; Functional Assays: Minimum Dilution: 0.1; Maximum Dilution: 10.0ng/ml; Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane. Researchers should empirically determine the suitability of the Il10 il10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-10, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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