GALECTIN-1 Active Protein | LGALS1 active protein

RECOMBINANT HUMAN GALECTIN-1

Cat. Num. AAA232407

• Gene Names: LGALS1; GBP; GAL1
• Applications: ELISA, Functional Assay, Western Blot
• Purity: >98% by SDS PAGE/HPLC analysis
• Synonyms: GALECTIN-1; RECOMBINANT HUMAN GALECTIN-1; LGALS1 active protein

• Purity/Purification: >98% by SDS PAGE/HPLC analysis
• Form/Format: Purified recombinant protein - lyphilized; 10mM Sodium phosphate pH7.5
• Concentration: Total protein concentration 0.1 mg/ml after reconstitution (varies by lot)
• Sequence Length: 135

• Applicable Applications for LGALS1 active protein: ELISA (EIA), Functional Assays (FN), Western Blot (WB)
• Application Notes: ELISA: Suggested Dilution: 0.2 - 0.4ng/well; Western Blotting: Suggested Dilution: 1.5 - 3.0ng/lane
• Reconstitution: Reconstitute with 0.5ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For extended storage, the additiona of 0.1% bovine serum albumin (BSA) is recommended.
• Preparation: Purified recombinant galectin-1 expressed in E Coli.
• Endotoxin Level: < 1 EU/ug
• Activity: Determined by its ability to chemoattract human blood monocytes. Chemotactic activity was observed at a concentration of 2.5 ug/ml with a peak response obtained at 250 ug/ml.
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4°C.; After reconstitution store at -20°C.; Storage in frost-free freezers is not recommended.; This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Related Product Information for LGALS1 active protein

Human galectin-1 (Gal-1) is a member of the galectin family of beta-galactoside-binding proteins, which exhibits both intracellular and extracellular functions, and is differentially expressed by a wide range of normal and neoplastic tissues. Galectin-1 has been implicated in several major cellular functions including proliferation, motility, haematopoietic cell differentiation, apoptosis promotion and immunoregulatory effects. The expression of Gal-1 by neuronal and glial cells correlates with neuroregeneration following injury, and studies have shown the importance of Gal-1 to primary sensory neurons and synaptic connections in the spinal cord, during embryonic development.

NCBI and Uniprot Product Information

• NCBI GI #: 4504981
• NCBI GeneID: 3956
• NCBI Accession #: NP_002296.1
• NCBI GenBank Nucleotide #: NM_002305.3
• UniProt Accession #: P09382; Q9UDK5; B2R5E8
• Molecular Weight: 14.5 kDa (134 amino acid residues)
• NCBI Official Full Name: galectin-1
• NCBI Official Synonym Full Names: lectin, galactoside-binding, soluble, 1
• NCBI Official Symbol: LGALS1
• NCBI Official Synonym Symbols: GBP; GAL1
• NCBI Protein Information: galectin-1; 14 kDa laminin-binding protein; 14 kDa lectin; HBL; HLBP14; HPL; S-Lac lectin 1; beta-galactoside-binding lectin L-14-I; beta-galactoside-binding protein 14kDa; gal-1; galaptin; galectin 1; lactose-binding lectin 1; putative MAPK-activating protein PM12
• UniProt Protein Name: Galectin-1
• Protein Family: Galectin
• UniProt Gene Name: LGALS1
• UniProt Synonym Gene Names: Gal-1; HLBP14
• UniProt Entry Name: LEG1_HUMAN

NCBI Description

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

Galectin-1: May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Homodimer. Binds LGALS3BP. Interacts with CD2, CD3, CD4, CD7, CD43 and CD45. Interacts with laminin (via poly-N- acetyllactosamine). Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion. Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.

Protein type: Cell adhesion

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; cell surface; cytoplasm; intracellular; nucleus

Molecular Function: protein binding; signal transducer activity; protein homodimerization activity; lactose binding; laminin binding; galactoside binding; glycoprotein binding

Biological Process: regulation of apoptosis; myoblast differentiation; response to drug; plasma cell differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; T cell costimulation; positive regulation of erythrocyte aggregation; response to axon injury; signal transduction; multicellular organismal response to stress