Rabbit anti-Human, Mouse Galectin-1 Polyclonal Antibody | anti-GAL1 antibody

Galectin-1 (GAL1, LGALS1, GAL-1, Lectin Galactoside-binding Soluble 1, Beta-galactoside-binding Lectin L-14-I, Lactose-binding Lectin 1, S-Lac Lectin 1, Galaptin, 14kD Laminin Binding Protein, 14kD Lectin, HLBP14, HPL, HBL, Putative MAPK-activating Protei

Cat. Num. AAA6379255

• Gene Names: LGALS1; GBP; GAL1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Galectin-1; Polyclonal Antibody; Galectin-1 (GAL1; LGALS1; GAL-1; Lectin Galactoside-binding Soluble 1; Beta-galactoside-binding Lectin L-14-I; Lactose-binding Lectin 1; S-Lac Lectin 1; Galaptin; 14kD Laminin Binding Protein; 14kD Lectin; HLBP14; HPL; HBL; Putative MAPK-activating Protei; anti-GAL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LGALS1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GAL1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human LGALS1, aa1-135 (NP_002296.1).
• Immunogen Sequence: MACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(LGALS1 rabbit polyclonal antibody. Western Blot analysis of LGALS1 expression in human kidney.)

Western Blot (WB)

(LGALS1 rabbit polyclonal antibody. Western Blot analysis of LGALS1 expression in mouse spleen.)

Western Blot (WB)

(LGALS1 rabbit polyclonal antibody. Western Blot analysis of LGALS1 expression in HeLa.)

Western Blot (WB)

(Western Blot analysis of LGALS1 expression in transfected 293T cell line by LGALS1 polyclonal antibody. Lane 1: LGALS1 transfected lysate (14.7kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-GAL1 antibody

Human galectin-1 (Gal-1), a member of the galectin family of beta-galactoside-binding proteins, which exhibits both intracellular and extracellular functions, and is differentially expressed by a wide range of normal and neoplastic tissues. Gal-1 has been implicated in several major cellular functions including proliferation, motility, hematopoietic cell differentiation, apoptosis promotion and immunoregulatory effects. The expression of Gal-1 by neuronal and glial cells correlates with neuroregeneration following injury, and studies have shown the importance of Gal-1 to primary sensory neurons and synaptic connections in the spinal cord, during embryonic development.

Product Categories/Family for anti-GAL1 antibody

Antibodies; Lamin; Laminin

References

1. Transgenic overexpression of the {alpha}7 integrin reduces muscle pathology and improves viability in the dyW mouse model of merosin-deficient congenital muscular dystrophy type 1A. Doe JA, Wuebbles RD, Allred ET, Rooney JE, Elorza M, Burkin DJ.J Cell Sci. 2011 Jul 1;124(Pt 13):2287-97. Epub 2011 Jun 7.

NCBI and Uniprot Product Information

• NCBI GI #: 4504981
• NCBI GeneID: 3956
• NCBI Accession #: NP_002296.1
• NCBI GenBank Nucleotide #: NM_002305
• UniProt Accession #: P09382; Q9UDK5; B2R5E8
• Molecular Weight: 14,716 Da
• NCBI Official Full Name: galectin-1
• NCBI Official Synonym Full Names: lectin, galactoside-binding, soluble, 1
• NCBI Official Symbol: LGALS1
• NCBI Official Synonym Symbols: GBP; GAL1
• NCBI Protein Information: galectin-1; HBL; HPL; gal-1; HLBP14; galaptin; galectin 1; 14 kDa lectin; S-Lac lectin 1; lactose-binding lectin 1; 14 kDa laminin-binding protein; putative MAPK-activating protein PM12; beta-galactoside-binding lectin L-14-I; beta-galactoside-binding pro
• UniProt Protein Name: Galectin-1
• Protein Family: Galectin
• UniProt Gene Name: LGALS1
• UniProt Synonym Gene Names: Gal-1; HLBP14
• UniProt Entry Name: LEG1_HUMAN

NCBI Description

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

Galectin-1: May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Homodimer. Binds LGALS3BP. Interacts with CD2, CD3, CD4, CD7, CD43 and CD45. Interacts with laminin (via poly-N- acetyllactosamine). Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion. Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.

Protein type: Cell adhesion

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; cell surface; cytoplasm; intracellular; nucleus

Molecular Function: protein binding; signal transducer activity; protein homodimerization activity; lactose binding; laminin binding; galactoside binding; glycoprotein binding

Biological Process: myoblast differentiation; response to drug; regulation of apoptosis; plasma cell differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; T cell costimulation; positive regulation of erythrocyte aggregation; response to axon injury; signal transduction; multicellular organismal response to stress