Fibroblast Growth Factor 9 Active Protein | FGF9 active protein

Fibroblast Growth Factor 9, Recombinant, Human (FGF9)

Cat. Num. AAA650984

• Gene Names: FGF9; GAF; SYNS3; HBFG-9; MGC119914; MGC119915
• Purity: Highly Purified; ~95% (SDS PAGE)
• Synonyms: Fibroblast Growth Factor 9; Recombinant; Human (FGF9); FGF9 active protein

• Purity/Purification: Highly Purified; ~95% (SDS PAGE)
• Form/Format: Lyophilized from 10 mM Tris, pH 8.0, 50mM sodium chloride. Reconstitute with 10mM Tris, pH 8.0, 50 mM sodium chloride to 0.1-1.0 mg/ml.

• Activity: ED50 <0.5 ng/ml (> 2 x 106units/mg), determined by the dose dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors. FGF-9 is a heparin binding growth factor that has principal activity in and on, nervous tissue. Cells known to express FGF-9 include prostatic stromal cells, neurons, astrocytes and oligodendrocytes and fetal skeletal myoblasts, ventricular myocardium, gut lumenal epithelium and olfactory bulb. It is also known as glial-activating factor (GAF).
• Endotoxin Level: 0.1ng/ug
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with Tris, pH 8.0, sodium chloride. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for FGF9 active protein

Growth Factors; Cytokines; Growth Factors-FGF

NCBI and Uniprot Product Information

• NCBI GI #: 391719
• NCBI GeneID: 2254
• UniProt Accession #: P31371; Q3SY32; A8K427
• Molecular Weight: 23.4kD, 207 amino acid residues
• NCBI Official Full Name: fibroblast growth factor 9
• NCBI Official Synonym Full Names: fibroblast growth factor 9 (glia-activating factor)
• NCBI Official Symbol: FGF9
• NCBI Official Synonym Symbols: GAF; SYNS3; HBFG-9; MGC119914; MGC119915
• NCBI Protein Information: glia-activating factor; FGF-9; HBGF-9; OTTHUMP00000018804; heparin-binding growth factor 9
• UniProt Protein Name: Glia-activating factor
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF9
• UniProt Entry Name: FGF9_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis. [provided by RefSeq]

Uniprot Description

Function: May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.

Subunit structure: Monomer.

Subcellular location: Secreted.

Tissue specificity: Glial cells.

Post-translational modification: Three molecular species were found (30 kDa, 29 kDa and 25 kDa), cleaved at Leu-4, Val-13 and Ser-34 respectively. The smaller ones might be products of proteolytic digestion. Furthermore, there may be a functional signal sequence in the 30 kDa species which is uncleavable in the secretion step.N-glycosylated.

Involvement in disease: Defects in FGF9 are the cause of multiple synostoses syndrome type 3 (SYNS3) [

MIM:612961]. Multiple synostoses syndrome is an autosomal dominant condition characterized by progressive joint fusions of the fingers, wrists, ankles and cervical spine, characteristic facies and progressive conductive deafness. Ref.9

Miscellaneous: Biochemical analysis of the Asn-99 mutation reveals a significantly impaired FGF signaling, as evidenced by diminished activity of the MAPK1/MAPK2 pathway and decreases CTNNB1 and MYC expression when compared with wild-type protein. Binding of mutant protein to the receptor FGFR3 is severely impaired, although homodimerization of mutant to itself or wild-type is not detectably affected, providing a basis for the observed defective FGF9 signaling.

Sequence similarities: Belongs to the heparin-binding growth factors family.