Fas Ligand Active Protein | FASLG active protein
Fas Ligand, Recombinant, Human (FASL) (BSA Free)
Purity
Highly Purified98% by SDS-PAGE
Synonyms
Fas Ligand; Recombinant; Human (FASL) (BSA Free); FASLG active protein
Purity/Purification
Highly Purified
98% by SDS-PAGE
98% by SDS-PAGE
Form/Format
Supplied as a lyophillized powder from PBS, pH 7.2.
Biological Activity
Determined by its ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is <10ng/ml, corresponding to a specific activity of >1x10e5 units/mg.
Endotoxin Level
0.1ng/ug (1EU/ug).
Reconstitution
Spin down product and reconstitute in water to a concentration of 0.1-1.0mg/ml.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for FASLG active protein
FasL is a type II membrane protein belonging to the TNF family of cytokines. FasL induces apoptosis when it binds to cells expressing the Fas antigen. FasL was first discovered on the cell surface of T cells and NK cells. Expressed on the cell surface of activated T cells, FasL kills T cells and activated B cells leading to down-regulation of the immune response. The full length of FasL contains 281 amino acid residues, consisting of the transmembrane domain, extracellular domain and the cytoplasmic region. A metalloproteinase cleaves the membrane-bound FasL to produce soluble FasL (sFasL), which exists as a homotrimer. The sFasL shows lower cytotoxic activity than membrane bound FasL. Recombinant Fas Ligand is a soluble 19.9kD protein containing 175 amino acid residues, comprising of only the extracellular domain of FasL.
Product Categories/Family for FASLG active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,410 Da
NCBI Official Full Name
Fas ligand
NCBI Official Symbol
FASLG
NCBI Protein Information
Fas ligand; CD95 ligand; APO-1 ligand; TNF superfamily, member 6
UniProt Protein Name
Fas ligand
Protein Family
UniProt Entry Name
Q9TTJ2_BOVIN
Research Articles on FASLG
Similar Products
Product Notes
The FASLG (Catalog #AAA650674) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Fas Ligand, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
