ELASTASE Active Protein | ELANE active protein

NATIVE HUMAN ELASTASE (NEUTROPHIL)

Cat. Num. AAA230127

• Gene Names: ELANE; GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
• Applications: ELISA
• Purity: >95% by SDS PAGE
• Synonyms: ELASTASE; NATIVE HUMAN ELASTASE (NEUTROPHIL); ELANE active protein

• Purity/Purification: >95% by SDS PAGE
• Form/Format: Purified; Purified protein from human neutrophils - liquid
• Concentration: Approximate Protein Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 267

• Applicable Applications for ELANE active protein: ELISA (EIA)
• Buffer Solution: 50mM sodium acetate, pH 5.5150mM sodium chloride
• Activity: 20-22 U/mg protein1 unit hydrolyzes one umole per minute of MeO-Suc-Ala-Ala-Pro-Val-pNA at 25 degree C, in 100mM Tris-Hcl, pH 7.5 with 500mM NaCl.
• Target Species: Human
• Preparation and Storage: Store at -20 degree C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of despatch.

Related Product Information for ELANE active protein

Human Elastase is a 28.5kDa serine protease that has been purified from human neutrophils. Elastase is also present in the pancreas, leucocytes and serum.

NCBI and Uniprot Product Information

• NCBI GI #: 4503549
• NCBI GeneID: 1991
• NCBI Accession #: NP_001963.1
• NCBI GenBank Nucleotide #: NM_001972.2
• UniProt Accession #: P08246; P09649; Q6B0D9; Q6LDP5
• Molecular Weight: 28.5 kDa
• NCBI Official Full Name: neutrophil elastase preproprotein
• NCBI Official Synonym Full Names: elastase, neutrophil expressed
• NCBI Official Symbol: ELANE
• NCBI Official Synonym Symbols: GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
• NCBI Protein Information: neutrophil elastase; PMN elastase; bone marrow serine protease; elastase 2, neutrophil; elastase-2; granulocyte-derived elastase; human leukocyte elastase; medullasin; polymorphonuclear elastase
• UniProt Protein Name: Neutrophil elastase
• Protein Family: Elastase
• UniProt Gene Name: ELANE
• UniProt Synonym Gene Names: ELA2; HLE
• UniProt Entry Name: ELNE_HUMAN

NCBI Description

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009]

Uniprot Description

ELANE: Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Defects in ELANE are a cause of cyclic haematopoiesis (CH); also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency. Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1). SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: EC 3.4.21.37; Motility/polarity/chemotaxis; Cell cycle regulation; Cell surface; Protease

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cell surface; transcriptional repressor complex; cytoplasm; extracellular region; secretory granule

Molecular Function: heparin binding; peptidase activity; protein binding; protease binding; cytokine binding; serine-type endopeptidase activity; endopeptidase activity

Biological Process: positive regulation of immune response; extracellular matrix organization and biogenesis; positive regulation of smooth muscle cell proliferation; negative regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-8 biosynthetic process; response to lipopolysaccharide; negative regulation of chemotaxis; negative regulation of transcription from RNA polymerase II promoter; proteolysis; phagocytosis; cellular calcium ion homeostasis; collagen catabolic process; positive regulation of MAP kinase activity; extracellular matrix disassembly; negative regulation of inflammatory response; defense response to bacterium; response to yeast; protein catabolic process; leukocyte migration; acute inflammatory response to antigenic stimulus; response to UV; negative regulation of chemokine biosynthetic process

Disease: Cyclic Neutropenia; Neutropenia, Severe Congenital, 1, Autosomal Dominant