CEACAM 6 Active Protein | CEACAM6 active protein

CEACAM 6, Recombinant, Human (Carcinoembryonic Antigen Related Cell Adhesion Molecule)

Cat. Num. AAA636744

• Gene Names: CEACAM6; NCA; CEAL; CD66c
• Purity: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: CEACAM 6; Recombinant; Human (Carcinoembryonic Antigen Related Cell Adhesion Molecule); CEACAM6 active protein

• Purity/Purification: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS.

• Activity: Measured by the ability of the immobilized protein to support the adhesion of 1 M calcium ionosphere treated human neutrophils. When 2x10e5 cells/well are added to CEACAM-6-coated plates (10ug/ml, 100ul/well), 35-60% of the cells will adhere after 20 minutes at 37 degree C.
• Endotoxin Level: 1 EU/ug of the protein as determined by the LAL method.
• Reconstitution: It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
• Preparation and Storage: Lyophilized samples are stable for up to twelve months at -20 degree C. Upon reconstitution, this protein, in the presence of a carrier protein, can be stored under sterile conditions at 2-8 degree C for one month or at -20 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Related Product Information for CEACAM6 active protein

Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM-6), previously called nonspecific crossreacting antigen (NCA) or CD66c, is one of seven human CEACAM family members within the immunoglobulin superfamily (1-4). In humans, CEACAMs include type I transmembrane proteins (CEACAM-1, -3, and -4) and GPI-linked molecules (CEACAM-5 through -8) (1). There is no human CEACAM-2. Human CEACAM-6 is a 90 kD, GPI-linked membrane protein that contains a 34 amino acid (aa) signal sequence, a 286 aa extracellular domain (ECD), and a 24 aa hydrophobic C-terminal propeptide. The GPI membrane anchor is attached at the C terminus following cleavage of the propeptide. CEACAM-6 contains one N-terminal V-type Ig-like domain (N domain), followed by two C2-type Ig-like domains (2-4). It shows considerable glycosylation, including (sialyl) Lewis X, which mediates binding to E-selectin, galectins and some bacterial fimbrae (1, 2). Mature human CEACAM-6 shows 84%, 85%, 80%, 87% and 51% aa identity to the equivalent extracellular regions of human CEACAMs 1, 5 (CEA) and 8, rhesus CEACAM-2, and bovine CEACAM-6, respectively. CEACAM-6 is expressed by granulocytes and their precursors. Activation enhances surface expression by mobilizing CEACAM-6 from storage in azurophilic granules (5, 6). It often shows aberrant expression in acute lymphocytic leukemias (10). CEACAM-6 is also expressed epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas and hyperplastic polyps (7, 8). Over-expression confers resistance to adhesion-related apoptosis (anoikis) in tumor cells (8, 9). CEACAM-6 is an intercellular adhesion molecule, forming both homotypic, and heterotypic bonds with CEACAM-1, -5 and -8 through interaction of the V-type Ig-like domains (11, 12). Cross-linking of neutrophil CEACAM-6 augments v 3 and 2 integrin-mediated adhesion, apparently by src and caveolin-mediated inside-out integrin activation (8, 13, 14).

Product Categories/Family for CEACAM6 active protein

Molecular Biology; MB-Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 40255013
• NCBI GeneID: 4680
• NCBI Accession #: NP_002474.3
• NCBI GenBank Nucleotide #: NM_002483.4
• UniProt Accession #: P40199; Q13774; Q14920; Q53XP7
• Molecular Weight: Based on N-terminal sequencing, the recombinant human CEACAM-6 starts at Lys 35 and has a calculated molecular mass of approximately 32.0kD. As a result of glycosylation, the recombinant protein migrates as an approximately 57-75kD protein in SDS-PAGE und
• NCBI Official Full Name: carcinoembryonic antigen-related cell adhesion molecule 6
• NCBI Official Synonym Full Names: carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen)
• NCBI Official Symbol: CEACAM6
• NCBI Official Synonym Symbols: NCA; CEAL; CD66c
• NCBI Protein Information: carcinoembryonic antigen-related cell adhesion molecule 6; normal cross-reacting antigen; non-specific crossreacting antigen
• UniProt Protein Name: Carcinoembryonic antigen-related cell adhesion molecule 6
• Protein Family: Carcinoembryonic antigen-related cell adhesion molecule
• UniProt Gene Name: CEACAM6
• UniProt Synonym Gene Names: NCA
• UniProt Entry Name: CEAM6_HUMAN

NCBI Description

Carcinoembryonic antigen (CEA; MIM 114890) is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma. An apparent lack of absolute cancer specificity for CEA probably results in part from the presence in normal and neoplastic tissues of antigens that share antigenic determinants with the 180-kD form of CEA (Barnett et al., 1988 [PubMed 3220478]). For background information on the CEA family of genes, see CEACAM1 (MIM 109770).[supplied by OMIM, Mar 2008]

Uniprot Description

CEACAM6: Belongs to the immunoglobulin superfamily. CEA family

Protein type: Membrane protein, GPI anchor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding

Biological Process: cell-cell signaling; blood coagulation; signal transduction; leukocyte migration