CD14 active protein
CD14, Recombinant, Human (Monocyte Differentiation Antigen CD14, Myeloid Cell-specific Leucine-rich Glycoprotein)
Purity
Purified~90-95% (SDS-PAGE)
Synonyms
CD14; Recombinant; Human (Monocyte Differentiation Antigen CD14; Myeloid Cell-specific Leucine-rich Glycoprotein); CD14 active protein
Purity/Purification
Purified
~90-95% (SDS-PAGE)
~90-95% (SDS-PAGE)
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 10ul ultra-pure dH2O.
Biological Activity
Inhibits binding of LPS to CD14. Upto to 20ug/ml CD14 inhibit binding of FITC-LPS (0.5ug/ml) to 6x10e5 CD14+CHO transfectants (FACS)
Endotoxin Level
<0.01ng/ml
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for CD14 active protein
CD14 acts as a receptor for endotoxin (LPS), is expressed strongly on monocytes, macro¬pha¬ges and weekly at surface of neutrophiles. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor LPS-LBP (lipopolysaccharide binding protein)-complexes. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids, acts at physiological concentrations as LPS agonist and has at higher concentrations an antagonizing effect in cell activation. The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wild type CD14 is the 50kD protein.
Product Categories/Family for CD14 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
~ 50kD
NCBI Official Full Name
CD14
NCBI Official Synonym Full Names
CD14 molecule<
NCBI Official Symbol
CD14
NCBI Protein Information
monocyte differentiation antigen CD14; CD14 antigen; myeloid cell-specific leucine-rich glycoprotein
UniProt Protein Name
Monocyte differentiation antigen CD14
Protein Family
UniProt Gene Name
CD14
UniProt Entry Name
CD14_BOVIN
Uniprot Description
Function: In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules
By similarity.
Subunit structure: Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4
By similarity.
Subcellular location: Cell membrane; Lipid-anchor › GPI-anchor
By similarity.
Sequence similarities: Contains 11 LRR (leucine-rich) repeats.
Research Articles on CD14
Similar Products
Product Notes
The CD14 cd14 (Catalog #AAA637301) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CD14, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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