c-Met, Canine Active Protein | MET active protein

c-Met, Recombinant, Canine (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR)

Cat. Num. AAA637402

• Gene Names: MET; c-Met
• Purity: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: c-Met; Canine; Recombinant; Canine (Hepatocyte Growth Factor Receptor; Scatter Factor Receptor; HGFR; SFR); MET active protein

• Purity/Purification: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS.

• Activity: Measured by its ability to bind rcaHGF with an estimated Kd < 0.8 nm
• Endotoxin Level: < 1.0 EU per 1 g of the protein as determined by the LAL method.
• Preparation and Storage: Lyophilized samples are stable for up to twelve months from date of receipt at -20 degree C. Upon reconstitution, this protein, in the presence of a carrier protein, can be stored under sterile conditions at 2-8 degree C for one month or at -20 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Related Product Information for MET active protein

HGF R, also known as Met (from N-methyl-N'-nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes posttranslational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kD extracellular chain and a 145 kD transmembrane chain. 1,2 The extracellular domain (ECD) contains a seven bladed - propeller sema domain, a cysteine-rich PSI/MRS region, and four Ig-like E-set domains, while the cytoplasmic region includes a tyrosine kinase domain. 3 The sema domain, which is formed by both the and chains of HGF R, mediates both ligand binding and receptor dimerization. 3,4 Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2-containing molecules. 5,6 HGF stimulation induces HGF R downregulation via internalization and proteasome-dependent degradation. 7 In the absence of ligand, HGF R forms noncovalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, integrin 6/ 4, plexins B1, B2, and B3, and MSP R/Ron. 8-15 Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects. 8-15 Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion. 8,12,13 HGF released from neighboring mesenchymal cells stimulates HGF R on undifferentiated epithelium and induces epithelial cell scattering and branching tubulogenesis. 16 Genetic polymorphisms, chromosomal translocation, overexpression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers. 1 Within the ECD, canine HGF R shares 85%-88% aa sequence identity with human, mouse and rat HGF R.

Product Categories/Family for MET active protein

Molecular Biology; MB-Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 25815188
• NCBI GeneID: 403438
• UniProt Accession #: Q75ZY9; A0M8U9
• Molecular Weight: The mature recombinant canine HGF R/His is a disulfide-linked heterodimer. Based on N-terminal amino acid sequencing, the recombinant canine HGF R/His has two N-termini starting at Glu 25 and Ser 309 that correspond to the and chains, respectively. The pr
• NCBI Official Full Name: c-Met
• NCBI Official Symbol: MET
• NCBI Official Synonym Symbols: c-Met
• NCBI Protein Information: hepatocyte growth factor receptor; SF receptor; HGF receptor; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase
• UniProt Protein Name: Hepatocyte growth factor receptor
• Protein Family: Hepatocyte growth factor receptor
• UniProt Gene Name: MET
• UniProt Synonym Gene Names: HGF receptor; SF receptor
• UniProt Entry Name: MET_CANFA

Uniprot Description

Function: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells

By similarity. Ref.4

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Enzyme regulation: In its inactive state, the C-terminal tail interacts with the catalytic domain and inhibits the kinase activity. Upon ligand binding, the C-terminal tail is displaced and becomes phosphorylated, thus increasing the kinase activity

By similarity.

Subunit structure: Heterodimer made of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1. Interacts when phosphorylated with downstream effectors including STAT3, PIK3R1, SRC, PCLG1, GRB2 and GAB1. Interacts with SPSB1, SPSB2 and SPSB4. Interacts with INPP5D/SHIP1. When phosphorylated at Tyr-1357, interacts with INPPL1/SHIP2. Interacts with RANBP9 and RANBP10, as well as SPSB1, SPSB2, SPSB3 and SPSB4. SPSB1 binding occurs in the presence and in the absence of HGF, however HGF treatment has a positive effect on this interaction. Interacts with MUC20; prevents interaction with GRB2 and suppresses hepatocyte growth factor-induced cell proliferation. Interacts with GRB10

By similarity. Interacts with PTPN1 and PTPN2

By similarity.

Subcellular location: Membrane; Single-pass type I membrane protein

By similarity.

Domain: The kinase domain is involved in SPSB1 binding

By similarity.The beta-propeller Sema domain mediates binding to HGF

By similarity.

Post-translational modification: Autophosphorylated in response to ligand binding on Tyr-1235 and Tyr-1236 in the kinase domain leading to further phosphorylation of Tyr-1350 and Tyr-1357 in the C-terminal multifunctional docking site. Dephosphorylated by PTPRJ at Tyr-1350 and Tyr-1366. Dephosphorylated by PTPN1 and PTPN2

By similarity.Ubiquitinated. Ubiquitination by CBL regulates the receptor stability and activity through proteasomal degradation

By similarity.

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family.Contains 3 IPT/TIG domains.Contains 1 protein kinase domain.Contains 1 Sema domain.