BETA GALACTOSIDASE Active Protein | lacZ active protein
NATIVE ESCHERICHIA COLI BETA GALACTOSIDASE
Gene Names
lacZ; ECK0341; JW0335
Applications
Functional Assay
Synonyms
BETA GALACTOSIDASE; NATIVE ESCHERICHIA COLI BETA GALACTOSIDASE; lacZ active protein
Form/Format
Purified protein from Escherichia coli - lyophilized
Concentration
Protein content is approximately 20-25% of total weight (varies by lot)
Sequence Length
1024
Applicable Applications for lacZ active protein
Functional Assays (FN)
Target Species
Bacterial
Reconstitution
Reconstitute with 1.0 ml sterile distilled water or aqueous buffer of choice. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. It is recommended that the vial is gently mixed after reconstitution.
Buffer Solution
Phosphate buffered saline
Preservative Stabilizers
Contains Sucrose
Activity
>600 U/mg protein
One unit hydrolyses 1 umole of p-nitrophenol-b-D-galactoside to p-nitrophenol per minute at 37ºC. For batch-specific activity, please inquire.
One unit hydrolyses 1 umole of p-nitrophenol-b-D-galactoside to p-nitrophenol per minute at 37ºC. For batch-specific activity, please inquire.
Preparation and Storage
Prior to reconstitution store at +4°C.
After reconstitution store at -20°C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the protein.
Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the protein.
Should this product contain a precipitate we recommend microcentrifugation before use.
Related Product Information for lacZ active protein
Escherichia coli Beta-Galactosidase is an inducible tetrameric enzyme coded by the lac Z gene of the lac operon that is often used as a reporter to assess the efficiency of transfection. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
540 kD
NCBI Official Full Name
beta-D-galactosidase
NCBI Official Symbol
lacZ
NCBI Official Synonym Symbols
ECK0341; JW0335
NCBI Protein Information
beta-D-galactosidase
UniProt Protein Name
Beta-galactosidase
Protein Family
UniProt Gene Name
lacZ
UniProt Synonym Gene Names
Beta-gal
UniProt Entry Name
BGAL_ECOLI
NCBI Description
Repressed during biofilm formation. [More information is available at EcoGene: EG10527]. galacotsidase is a metalloenzyme exhibiting broad substrate specificity. [More information is available at EcoCyc: EG10527].
Uniprot Description
lacZ: Repressed during biofilm formation. [More information is available at EcoGene: EG10527]. galacotsidase is a metalloenzyme exhibiting broad substrate specificity. [More information is available at EcoCyc: EG10527]
Protein type: EC 3.2.1.23
Research Articles on lacZ
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Product Notes
The lacZ lacz (Catalog #AAA230338) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's BETA GALACTOSIDASE can be used in a range of immunoassay formats including, but not limited to, Functional Assays (FN). Researchers should empirically determine the suitability of the lacZ lacz for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BETA GALACTOSIDASE, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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