Typical Testing Data/Standard Curve (for reference only)
Human ACTalpha1 (Actin Alpha 1, Skeletal Muscle) CLIA Kit | ACTa1 clia kit
Human ACTalpha1 (Actin Alpha 1, Skeletal Muscle) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to ACTalpha1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ACTalpha1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ACTalpha1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of ACTalpha1. You can calculate the concentration of ACTalpha1 in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]
Uniprot Description
ACTA1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID. Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25m in muscle differentiating cells. Belongs to the actin family.
Protein type: Contractile; Cell development/differentiation; Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 1q42.13
Cellular Component: extracellular space; sarcomere; lamellipodium; stress fiber; actin filament; cytosol; striated muscle thin filament; actin cytoskeleton; filopodium
Molecular Function: protein binding; myosin binding; structural constituent of cytoskeleton; ADP binding; ATP binding
Biological Process: skeletal muscle fiber adaptation; muscle contraction; response to mechanical stimulus; response to steroid hormone stimulus; muscle thin filament assembly; response to extracellular stimulus; response to lithium ion; skeletal muscle fiber development; cell growth; muscle filament sliding
Disease: Nemaline Myopathy 3; Myopathy, Congenital, With Fiber-type Disproportion
Research Articles on ACTa1
Similar Products
Product Notes
The Human ACTa1 acta1 (Catalog #AAA2532453) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2532453 CLIA Kit recognizes Human ACTa1. It is sometimes possible for the material contained within the vial of "ACTalpha1 (Actin Alpha 1, Skeletal Muscle), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
