Typical Testing Data/Standard Curve (for reference only)
Chicken Anti-acrosin antibodies ELISA Kit | ACR elisa kit
Chicken Anti-acrosin antibodies ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This ACRAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken ACRAb. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY ACRAb ELISA kit applies the competitive enzyme immunoassay technique utilizing ACR antigen and an ACRAb-HRP conjugate. The assay sample and buffer are incubated together with ACRAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACRAb concentration since ACRAb from samples and ACRAb-HRP conjugate compete for the ACR antigen binding site. Since the number of sites is limited, as more sites are occupied by ACRAb from the sample, fewer sites are left to bind ACRAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACRAb concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids. [provided by RefSeq, Jul 2008]
Uniprot Description
ACR: Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome. Belongs to the peptidase S1 family.
Protein type: Protease; EC 3.4.21.10
Chromosomal Location of Human Ortholog: 22q13.33
Cellular Component: protein complex; extracellular region; Golgi-associated vesicle; nucleus; acrosomal matrix
Molecular Function: mannose binding; protein binding; copper ion binding; amidase activity; DNA binding; zinc ion binding; serine-type endopeptidase activity; drug binding; fucose binding
Biological Process: binding of sperm to zona pellucida; response to steroid hormone stimulus; single fertilization; adenylate cyclase activation; acrosome reaction; penetration of zona pellucida; acrosome matrix dispersal; multicellular organism reproduction
Research Articles on ACR
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Product Notes
The Chicken ACR acr (Catalog #AAA738477) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA738477 ELISA Kit recognizes Chicken ACR. It is sometimes possible for the material contained within the vial of "Anti-acrosin antibodies, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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