Aconitase-1 Recombinant Protein | ACO1 recombinant protein

Recombinant Human Aconitase-1

Cat. Num. AAA144183

• Gene Names: ACO1; IRP1; ACONS; HEL60; IREB1; IREBP; IREBP1
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Aconitase-1; Recombinant Human Aconitase-1; ACO1 Human; Aconitase-1 Human Recombinant; Onitase 1 Soluble; IRP1; IREB1; IREBP; Citrate hydro-lyase; Iron regulatory protein 1; Ferritin repressor protein; Iron-responsive element-binding protein 1; ACONS; Aconitate Hydratase; EC 4.2.1.3; Aconitase; ACO1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: The ACO1 protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0)2mM DTT, 100mM NaCl and 10% glycerol.; Sterile Filtered clear solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSNPFAH LAEPLDPVQP GKKFFNLNKL EDSRYGRLPF SIRVLLEAAI RNCDEFLVKK QDIENILHWN VTQHKNIEVP FKPARVILQD FTGVPAVVDF AAMRDAVKKL GGDPEKINPV CPADLVIDHS IQVDFNRRAD SLQKNQDLEF ERNRERFEFL KWGSQAFHNM RIIPPGSGII HQVNLEYLAR VVFDQDGYYY PDSLVGTDSH TTMIDGLGIL GWGVGGIEAE AVMLGQPISM VLPQVIGYRL MGKPHPLVTS TDIVLTITKH LRQVGVVGKF VEFFGPGVAQ LSIADRATIA NMCPEYGATA AFFPVDEVSI TYLVQTGRDE EKLKYIKKYL QAVGMFRDFN DPSQDPDFTQ VVELDLKTVV PCCSGPKRPQ DKVAVSDMKK DFESCLGAKQ GFKGFQVAPE HHNDHKTFIY DNTEFTLAHG SVVIAAITSC TNTSNPSVML GAGLLAKKAV DAGLNVMPYI KTSLSPGSGV VTYYLQESGV MPYLSQLGFD VVGYGCMTCI GNSGPLPEPV VEAITQGDLV AVGVLSGNRN FEGRVHPNTR ANYLASPPLV IAYAIAGTIR IDFEKEPLGV NAKGQQVFLK DIWPTRDEIQ AVERQYVIPG MFKEVYQKIE TVNESWNALA TPSDKLFFWN SKSTYIKSPP FFENLTLDLQ PPKSIVDAYV LLNLGDSVTT DHISPAGNIA RNSPAARYLT NRGLTPREFN SYGSRRGNDA VMARGTFANI RLLNRFLNKQ APQTIHLPSG EILDVFDAAE RYQQAGLPLI VLAGKEYGAG SSRDWAAKGP FLLGIKAVLA ESYERIHRSN LVGMGVIPLE YLPGENADAL GLTGQERYTI IIPENLKPQM KVQVKLDTGK TFQAVMRFDT DVELTYFLNG GILNYMIRKM AK
• Sequence Length: 889

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.

Related Product Information for ACO1 recombinant protein

Description: ACO1 produced in E Coli is a single, non-glycosylated polypeptide chain containing 912 amino acids (1-889a.a.) and having a molecular mass of 100.8kDa.ACO1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: ACO1 has a part in an iron sensor. ACO1 catalyzes the stereo-specific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process.

Product Categories/Family for ACO1 recombinant protein

Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 507834063
• NCBI GeneID: 48
• NCBI Accession #: NP_001265281.1
• NCBI GenBank Nucleotide #: NM_001278352.1
• UniProt Accession #: P21399; Q14652; Q5VZA7; D3DRK7
• Molecular Weight: 98,399 Da
• NCBI Official Full Name: cytoplasmic aconitate hydratase
• NCBI Official Synonym Full Names: aconitase 1, soluble
• NCBI Official Symbol: ACO1
• NCBI Official Synonym Symbols: IRP1; ACONS; HEL60; IREB1; IREBP; IREBP1
• NCBI Protein Information: cytoplasmic aconitate hydratase; IRE-BP 1; aconitate hydratase, cytoplasmic; citrate hydro-lyase; epididymis luminal protein 60; ferritin repressor protein; iron regulatory protein 1; iron-responsive element binding protein 1; iron-responsive element-binding protein 1
• UniProt Protein Name: Cytoplasmic aconitate hydratase
• Protein Family: Aconitate hydratase
• UniProt Gene Name: ACO1
• UniProt Synonym Gene Names: IREB1; Aconitase; IRP1; IRE-BP 1
• UniProt Entry Name: ACOC_HUMAN

NCBI Description

The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014]

Uniprot Description

IREB1: Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding. Belongs to the aconitase/IPM isomerase family.

Protein type: Translation; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Lyase; Carbohydrate Metabolism - citrate (TCA) cycle; RNA-binding; EC 4.2.1.3; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 9p21.1

Cellular Component: Golgi apparatus; mitochondrion; endoplasmic reticulum; cytoplasm; cytosol

Molecular Function: protein binding; iron-responsive element binding; RNA binding; metal ion binding; 4 iron, 4 sulfur cluster binding; aconitate hydratase activity

Biological Process: regulation of translation; cellular iron ion homeostasis; tricarboxylic acid cycle; citrate metabolic process; response to iron(II) ion; intestinal absorption; post-embryonic development