ATP-Citrate Lyase Blocking Peptide | ACL blocking peptide

Phospho-ATP citrate lyase (Thr447) Blocking Peptide

Cat. Num. AAA9618403

• Gene Names: ACLY; ACL; ATPCL; CLATP
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: ATP-Citrate Lyase; Phospho-ATP citrate lyase (Thr447) Blocking Peptide; Acly; ACLY_HUMAN; ATP citrate (pro-S) lyase; ATP citrate lyase; ATP citrate synthase; ATP-citrate (pro-S-)-lyase; ATP-citrate synthase; ATPcitrate synthase; ATPCL; Citrate cleavage enzyme; CLATP; OTTHUMP00000164773; ACL blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for ACL blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ACL blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 38569421
• NCBI GeneID: 47
• NCBI Accession #: NP_001087.2
• NCBI GenBank Nucleotide #: NP_001087.2
• UniProt Accession #: P53396; Q13037; Q9BRL0; B4DIM0
• Molecular Weight: 120,839 Da
• NCBI Official Full Name: ATP-citrate synthase isoform 1
• NCBI Official Synonym Full Names: ATP citrate lyase
• NCBI Official Symbol: ACLY
• NCBI Official Synonym Symbols: ACL; ATPCL; CLATP
• NCBI Protein Information: ATP-citrate synthase; ATP citrate synthase; citrate cleavage enzyme; ATP-citrate (pro-S-)-lyase
• UniProt Protein Name: ATP-citrate synthase
• UniProt Gene Name: ACLY
• UniProt Synonym Gene Names: ACL
• UniProt Entry Name: ACLY_HUMAN

NCBI Description

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ACLY: ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine. Homotetramer. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.3.8; Transferase; Carbohydrate Metabolism - citrate (TCA) cycle; Lyase

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: nucleoplasm; membrane; mitochondrion; citrate lyase complex; cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; ATP citrate synthase activity; metal ion binding; succinate-CoA ligase (ADP-forming) activity; cofactor binding; ATP binding

Biological Process: coenzyme A metabolic process; lipid biosynthetic process; positive regulation of cellular metabolic process; energy reserve metabolic process; citrate metabolic process; cellular carbohydrate metabolic process; triacylglycerol biosynthetic process; cellular lipid metabolic process