ACE2 recombinant protein

Recombinant Human ACE2 protein

Cat. Num. AAA156033

• Applications: Immunogen
• Purity: >90% as determined by SDS-PAGE quantitative densitometry by Coomasssie Blue staining
• Synonyms: ACE2; Recombinant Human ACE2 protein; ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; Metalloprotease MPROT15; ACE2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE quantitative densitometry by Coomasssie Blue staining
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 5% Trehalose, 5% Mannose?1mM EDTA
• Sequence Length: 805

• Applicable Applications for ACE2 recombinant protein: Immunogen
• Species: Homo sapiens (Human)
• Reconstiution: Reconstitute in ddH2O to a concentration of 0.1-1.0mg/ml. Do not vortex.
• Preparation and Storage: Store at 2 to 8 degree C for one week.; Store at -20 to -80 degree C for twelve months from the date of receipt.; Use a manual defrost freezer and avoid repeated freeze thaw cycles.; Shipping: Ice packs

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Related Product Information for ACE2 recombinant protein

Recombinant Human ACE2 is produced by HEK293 cells expression system and the target gene encoding Met1-Ser740 is expressed with C-His Tag

NCBI and Uniprot Product Information

• NCBI GI #: 1700998532
• NCBI Accession #: NP_001358344.1
• NCBI GenBank Nucleotide #: NP_001358344.1
• UniProt Accession #: Q9BYF1
• Molecular Weight: Actual Molecular Mass: 100-110kDa; Predicted Molecular Mass: 86.28kDa
• NCBI Official Full Name: angiotensin-converting enzyme 2
• UniProt Protein Name: Angiotensin-converting enzyme 2
• Protein Family: Angiotensin-converting enzyme
• UniProt Gene Name: ACE2
• UniProt Synonym Gene Names: ACEH
• UniProt Entry Name: ACE2_HUMAN

Uniprot Description

ACE2: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin- 13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. Interacts with ITGB1. Interacts with SARS-CoV and HCoV- NL63 spike glycoprotein. Up-regulated in failing heart. Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells. Expressed in heart, kidney, testis, and gastrointestinal system. Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. Belongs to the peptidase M2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.17.23; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: Xp22

Cellular Component: extracellular space; cell surface; cytoplasm; integral to membrane; plasma membrane; extracellular region; lipid raft

Molecular Function: peptidyl-dipeptidase activity; viral receptor activity; protein binding; metallopeptidase activity; carboxypeptidase activity; zinc ion binding; peptide hormone binding; endopeptidase activity; glycoprotein binding

Biological Process: virion attachment, binding of host cell surface receptor; entry of virus into host cell; maternal process involved in pregnancy; regulation of vasodilation; proteolysis; angiotensin maturation; regulation of systemic arterial blood pressure by renin-angiotensin; regulation of cell proliferation; regulation of cytokine production; cellular protein metabolic process; regulation of vasoconstriction; regulation of inflammatory response; angiotensin catabolic process in blood; receptor biosynthetic process; angiotensin mediated drinking behavior