Testing Data #2

MOUSE SEROBLOCK FCR Accessory Reagent

MOUSE SEROBLOCK FcR

Applications

Flow Cytometry, Functional Assay

Synonyms

MOUSE SEROBLOCK FCR; MOUSE SEROBLOCK FcR; MOUSE SEROBLOCK FCR accessory reagent

Ordering
Cat. Num. AAA238295 For Research Use Only!

Isotype

IgG2b

Clone Number

FCR4G8

Form/Format

Reagent
Purified IgG - liquid

Concentration

IgG concentration 1.0mg/ml (varies by lot)

Applicable Applications for MOUSE SEROBLOCK FCR accessory reagent

Flow cytometry (FC/FACS)

Perservative Stabilisers

0.09% Sodium Azide (NaN3)
Preparation

Immunogen

PU5 1.8 IOE7 Balb/c mouse cell line
Buffer Solution

Target Species

Mouse

Preparation and Storage

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Testing Data #2

(Hamster anti Mouse TCR Gamma/DeltaRPE antibody (MBS213590) staining mouse spleen cells in the absence (blue line) and presence (red histogram) of SeroBlock FcR (MBS238296))

Related Product Information for MOUSE SEROBLOCK FCR accessory reagent

MBS238295 is a rat antibody that recognises mouse CD16 and CD32, cell surface proteins also known as FcRgammaIII and FcRgammaII, respectively. The function of these proteins is to bind IgG molecules via their Fc regions as part of the adaptive immune response. CD16 and/or CD32 are expressed by a wide variety of cells, including monocytes, macrophages, B lymphocytes, granulocytes, NK cells, dendritic cells and some activated T lymphocytes. The expression of CD16/CD32 antigens can lead to non-specific binding of test monoclonal antibodies in staining procedures, resulting in œhigh background staining on a wide range of cells. This non-specific binding may be blocked by pre-incubation of target cells with MBS238295, resulting in clearer staining. For direct analysis of CD16/32 expression this antibody is also available conjugated directly to FITC (MBS210943), AlexaFluor488 (MBS212682A488), AlexaFluor647 (MBS212682A647), Biotin (MBS210447) and RPE (MBS213784).

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Product Notes

The MOUSE SEROBLOCK FCR (Catalog #AAA238295) is an Accessory Reagent and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MOUSE SEROBLOCK FCR can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Researchers should empirically determine the suitability of the MOUSE SEROBLOCK FCR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MOUSE SEROBLOCK FCR, Accessory Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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MOUSE SEROBLOCK FCR Accessory Reagent