ACAT2 recombinant protein

ACAT2 Protein, Rat, Recombinant (His Tag)

Cat. Num. AAA8123491

• Gene Names: Acat2; Acat3; Ab2-076
• Purity: >95% as determined by SDS-PAGE
• Synonyms: ACAT2; ACAT2 Protein; Rat; Recombinant (His Tag); Rat ACAT2 Protein (His Tag); acetyl-CoA acetyltransferase 2; ACAT2 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 20% glycerol, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly397

• Species: Rat
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the rat Acat2 (NP_001006996.1)(Met1-Gly397) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ACAT2 recombinant protein

Background: Acyl-coenzyme A: cholesterol acyltransferase (ACAT) is an intracellular enzyme that produces cholesteryl esters in various tissues. In mammals, two ACAT genes (ACAT1 and ACAT2) have been identified. Together, these two enzymes are involved in storing cholesteryl esters as lipid droplets, in macrophage foam-cell formation, in absorbing dietary cholesterol, and in supplying cholesteryl esters as part of the core lipid for lipoprotein synthesis and assembly. The key difference in tissue distribution of ACAT1 and ACAT2 between humans, mice and monkeys is that, in adult human liver (including hepatocytes and bile duct cells), the major enzyme is ACAT1, rather than ACAT2. There is compelling evidence implicating a role for ACAT1 in macrophage foam-cell formation, and for ACAT2 in intestinal cholesterol absorption.Ubiquitin linkage to cysteine is an unconventional modification targeting protein for degradation. However, the physiological regulation of cysteine ubiquitylation is still mysterious. Here we found that ACAT2, a cellular enzyme converting cholesterol and fatty acid to cholesteryl esters, was ubiquitylated on Cys277 for degradation when the lipid level was low. gp78-Insigs catalysed Lys48-linked polyubiquitylation on this Cys277. A high concentration of cholesterol and fatty acid, however, induced cellular reactive oxygen species (ROS) that oxidized Cys277, resulting in ACAT2 stabilization and subsequently elevated cholesteryl esters. Furthermore, ACAT2 knockout mice were more susceptible to high-fat diet-associated insulin resistance. By contrast, expression of a constitutively stable form of ACAT2 (C277A) resulted in higher insulin sensitivity. ACAT2 is an appealing target for therapy to reduce coronary heart disease.

NCBI and Uniprot Product Information

• NCBI GI #: 55741502
• NCBI GeneID: 308100
• NCBI Accession #: NP_001006996.1
• NCBI GenBank Nucleotide #: NM_001006995.1
• UniProt Accession #: Q5XI22
• Molecular Weight: 41,108 Da
• NCBI Official Full Name: acetyl-CoA acetyltransferase, cytosolic
• NCBI Official Synonym Full Names: acetyl-CoA acetyltransferase 2
• NCBI Official Symbol: Acat2
• NCBI Official Synonym Symbols: Acat3; Ab2-076
• NCBI Protein Information: acetyl-CoA acetyltransferase, cytosolic; cytosolic acetoacetyl-CoA thiolase; acetyl-Coenzyme A acetyltransferase 2; acetyl-Coenzyme A acetyltransferase 3
• UniProt Protein Name: Acetyl-CoA acetyltransferase, cytosolic
• Protein Family: Acetyl-CoA acetyltransferase
• UniProt Gene Name: Acat2
• UniProt Entry Name: THIC_RAT

Uniprot Description

ACAT2: The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. [provided by RefSeq, Jul 2008]

Protein type: Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - valine, leucine and isoleucine degradation; EC 2.3.1.9; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Acetyltransferase; Amino Acid Metabolism - tryptophan; Lipid Metabolism - synthesis and degradation of ketone bodies; Lipid Metabolism - fatty acid; Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - lysine degradation

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleolus; cytosol; nucleus

Molecular Function: acetyl-CoA C-acetyltransferase activity; transferase activity, transferring groups other than amino-acyl groups

Biological Process: metabolic process; ketone body catabolic process; cellular response to nutrient; brain development; liver development