Acyl Coenzyme A Dehydrogenase, Short/Branched Chain Recombinant Protein | ACADSB recombinant protein

Recombinant Acyl Coenzyme A Dehydrogenase, Short/Branched Chain (ACADSB)

Cat. Num. AAA2123761

• Applications: SDS-Page, Western Blot
• Purity: >90%
• Synonyms: Acyl Coenzyme A Dehydrogenase; Short/Branched Chain; Recombinant Acyl Coenzyme A Dehydrogenase; Short/Branched Chain (ACADSB); SBCAD; ACAD7; 2-methyl branched chain acyl-CoA dehydrogenase; 2-methylbutyryl-coenzyme A dehydrogenase; ACADSB recombinant protein

• Host: E Coli
• Purity/Purification: >90%
• Form/Format: Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Sequence Positions: Met1~Ala298
• Sequence Length: 432

• Applicable Applications for ACADSB recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Source: Prokaryotic expression
• Species: Rat
• Tag: N-terminal His Tag
• Subcellular Location: Mitochondrion
• Isoelectric Point: 8
• Reconstitution: Reconstitute in PBS or others.
• Preparation and Storage: Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.; Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Product Categories/Family for ACADSB recombinant protein

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 6978433
• NCBI GeneID: 25618
• NCBI Accession #: NP_037216.1
• NCBI GenBank Nucleotide #: NP_037216.1
• UniProt Accession #: P70584
• Molecular Weight: Predicted Molecular Mass: 36.4kDa; Accurate Molecular Mass: 36kDa
• NCBI Official Full Name: short/branched chain specific acyl-CoA dehydrogenase, mitochondrial
• NCBI Official Synonym Full Names: acyl-CoA dehydrogenase, short/branched chain
• NCBI Official Symbol: Acadsb
• NCBI Protein Information: short/branched chain specific acyl-CoA dehydrogenase, mitochondrial
• UniProt Protein Name: Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial
• Protein Family: Short/branched chain specific acyl-CoA dehydrogenase
• UniProt Gene Name: Acadsb
• UniProt Synonym Gene Names: SBCAD; 2-MEBCAD; 2-methylbutyryl-CoA dehydrogenase
• UniProt Entry Name: ACDSB_RAT

NCBI Description

catalyzes the alpha, beta-dehydrogenation of valproyl-CoA and other acyl-CoA esters in fatty acid metabolism [RGD, Feb 2006]

Uniprot Description

ACADSB: Has greatest activity toward short branched chain acyl- CoA derivative such as (s)-2-methylbutyryl-CoA, isobutyryl-CoA, and 2-methylhexanoyl-CoA as well as toward short straight chain acyl-CoAs such as butyryl-CoA and hexanoyl-CoA. Can use valproyl- CoA as substrate and may play a role in controlling the metabolic flux of valproic acid in the development of toxicity of this agent. Defects in ACADSB are the cause of short/branched-chain acyl-CoA dehydrogenase deficiency (SBCADD); also known as 2-methylbutyryl-CoA dehydrogenase deficiency or 2- methylbutyryl glycinuria. SBCADD is an autosomal recessive disorder and consists of a defect in catabolism of L-isoleucine which is characterized by an increase of 2-methylbutyrylglycine and 2-methylbutyrylcarnitine in blood and urine. Affected individuals have seizures and psychomotor delay as the main clinical features. Belongs to the acyl-CoA dehydrogenase family.

Protein type: Oxidoreductase; EC 1.3.8.5; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Lipid Metabolism - fatty acid; Mitochondrial

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: short-branched-chain-acyl-CoA dehydrogenase activity; acyl-CoA dehydrogenase activity; electron carrier activity; FAD binding

Biological Process: acyl-CoA metabolic process; fatty acid beta-oxidation; fatty acid metabolic process