Typical Standard Curve/Testing Data
Rat Androgen binding protein ELISA Kit | ABP elisa kit
Rat Androgen binding protein ELISA Kit
Gene Names
Scgb2b3; Abpbg3; Gm4362
Reactivity
Rat
Synonyms
Androgen binding protein; Rat Androgen binding protein ELISA Kit; ABP elisa kit
Reactivity
Rat
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
100-2500 pg/mL
Sensitivity
1.0pg/ml
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for ABP elisa kit
Intended Uses: This ABP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ABP. This ELISA kit for research use only!
Principle of the Assay: ABP ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ABP antibody and an ABP-HRP conjugate. The assay sample and buffer are incubated together with ABP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ABP concentration since ABP from samples and ABP-HRP conjugate compete for the anti-ABP antibody binding site. Since the number of sites is limited, as more sites are occupied by ABP from the sample, fewer sites are left to bind ABP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ABP concentration in each sample is interpolated from this standard curve.
Principle of the Assay: ABP ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ABP antibody and an ABP-HRP conjugate. The assay sample and buffer are incubated together with ABP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ABP concentration since ABP from samples and ABP-HRP conjugate compete for the anti-ABP antibody binding site. Since the number of sites is limited, as more sites are occupied by ABP from the sample, fewer sites are left to bind ABP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ABP concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ABP elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
androgen-binding protein
NCBI Official Synonym Full Names
secretoglobin, family 2B, member 3
NCBI Official Symbol
Scgb2b3
NCBI Official Synonym Symbols
Abpbg3; Gm4362
NCBI Protein Information
androgen-binding protein
Similar Products
Product Notes
The Rat ABP (Catalog #AAA753073) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA753073 ELISA Kit recognizes Rat ABP. It is sometimes possible for the material contained within the vial of "Androgen binding protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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