Multidrug Resistance Associated Protein 1 (MRP1) Recombinant Protein | MRP1 recombinant protein

Recombinant Multidrug Resistance Associated Protein 1 (MRP1)

Cat. Num. AAA2018351

• Gene Names: Abcc1; MRP; Mrp1; Mdrap; Abcc1a; Abcc1b
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Multidrug Resistance Associated Protein 1 (MRP1); Recombinant Multidrug Resistance Associated Protein 1 (MRP1); MRP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- RH SLSAGLVGLS VSYSLQITAY LNWLVRMSSE METNIVAVER LKEYSETEKE APWQIQETAP PSTWPHSGRV EFRDYCLRYR EDLDLVLKHI NVTIEGGEKV GIVGRTGAGK SSLTLGLFRI NESAEGEIII DGVNIAKIGL HNLRFKITII PQDPVLFSGS LRMNLDPFSQ YSDEEVWMAL ELAHLKGFVS ALPDKLNHECAEGGENLSVG QRQLVCLARA LLRKTKILVL DEATAAVDLE TDNLIQSTI
• Sequence Length: 1528

• Applicable Applications for MRP1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Arg1219~Ile1469 (Accession # O35379) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Cell membrane; Multi-pass membrane protein
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 5.9
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6678848
• NCBI GeneID: 17250
• NCBI Accession #: NP_032602.1
• NCBI GenBank Nucleotide #: NM_008576.3
• UniProt Accession #: O35379
• Molecular Weight: 31.7kDa
• NCBI Official Full Name: multidrug resistance-associated protein 1
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family C (CFTR/MRP), member 1
• NCBI Official Symbol: Abcc1
• NCBI Official Synonym Symbols: MRP; Mrp1; Mdrap; Abcc1a; Abcc1b
• NCBI Protein Information: multidrug resistance-associated protein 1; LTC4 transporter; leukotriene C(4) transporter; multiple drug resistance-associated protein; ATP-binding cassette, sub-family C (CFTR/MRP), member 1a; ATP-binding cassette, sub-family C (CFTR/MRP), member 1b
• UniProt Protein Name: Multidrug resistance-associated protein 1
• Protein Family: 37S ribosomal protein
• UniProt Gene Name: Abcc1
• UniProt Synonym Gene Names: Abcc1a; Abcc1b; Mdrap; Mrp; LTC4 transporter
• UniProt Entry Name: MRP1_MOUSE

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein plays an essential role in the defense against toxic compounds and serves as the major high-affinity transporter of leukotriene C4. The encoded protein may also play an essential role in steroid hormone homeostasis as a transporter for steroid hormones and their metabolites. [provided by RefSeq, Nov 2011]

Uniprot Description

ABCC1: a multi-pass membrane protein. A multidrug resistance member of the ABC transporter family that confers resistance to anticancer drugs. A multi-pass membrane protein. Exports sphingosine-1-phosphate (S1P), a potent inflammatory mediator, from mast cells during mast cell-mediated immune responses. Nine alternatively spliced human isoforms have been reported.

Protein type: Transporter, ABC family; Membrane protein, multi-pass; Membrane protein, integral; Transporter

Cellular Component: membrane; basolateral plasma membrane; integral to plasma membrane; cytoplasm; integral to membrane; plasma membrane

Molecular Function: lipid-transporting ATPase activity; hydrolase activity; transporter activity; ATPase activity; nucleotide binding; long-chain fatty acid transporter activity; sphingolipid transporter activity; glutathione S-conjugate-exporting ATPase activity; xenobiotic-transporting ATPase activity; efflux transmembrane transporter activity; ATPase activity, coupled to transmembrane movement of substances; drug transporter activity; ATP binding

Biological Process: daunorubicin transport; response to drug; phospholipid efflux; transport; drug export; multidrug transport; xenobiotic transport; response to oxidative stress; plasma membrane long-chain fatty acid transport; transmembrane transport; positive regulation of cell migration