Rat Multidrug resistance-associated protein 1 ELISA Kit | Abcc1 elisa kit

Rat Multidrug resistance-associated protein 1 ELISA Kit

Cat. Num. AAA764647

• Gene Names: ABCC1; MRP; ABCC; GS-X; MRP1; ABC29
• Reactivity: Rat
• Synonyms: Multidrug resistance-associated protein 1; Rat Multidrug resistance-associated protein 1 ELISA Kit; Abcc1/ATP-binding cassette sub-family C member 1/Leukotriene C (4) transporter (LTC4 transporter)/; Abcc1 elisa kit

• Reactivity: Rat
• Sequence Length: 1531

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Abcc1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Abcc1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Abcc1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Abcc1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Abcc1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 134142337
• NCBI GeneID: 4363
• NCBI Accession #: NP_004987.2
• NCBI GenBank Nucleotide #: NM_004996.3
• Molecular Weight: 172,640 Da
• NCBI Official Full Name: multidrug resistance-associated protein 1
• NCBI Official Synonym Full Names: ATP binding cassette subfamily C member 1
• NCBI Official Symbol: ABCC1
• NCBI Official Synonym Symbols: MRP; ABCC; GS-X; MRP1; ABC29
• NCBI Protein Information: multidrug resistance-associated protein 1
• UniProt Protein Name: Multidrug resistance-associated protein 1
• Protein Family: Multidrug resistance-associated protein
• UniProt Gene Name: ABCC1
• UniProt Synonym Gene Names: MRP; MRP1; LTC4 transporter
• UniProt Entry Name: MRP1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a multispecific organic anion transporter, with oxidized glutatione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Alternatively spliced variants of this gene have been described but their full-length nature is unknown. [provided by RefSeq, Apr 2012]

Uniprot Description

ABCC1: a multi-pass membrane protein. A multidrug resistance member of the ABC transporter family that confers resistance to anticancer drugs. A multi-pass membrane protein. Exports sphingosine-1-phosphate (S1P), a potent inflammatory mediator, from mast cells during mast cell-mediated immune responses. Nine alternatively spliced human isoforms have been reported.

Protein type: Transporter; Transporter, ABC family; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p13.1

Cellular Component: basolateral plasma membrane; integral to plasma membrane; membrane; plasma membrane

Molecular Function: ATP binding; ATPase activity; ATPase activity, coupled to transmembrane movement of substances; transporter activity

Biological Process: arachidonic acid metabolic process; cobalamin metabolic process; leukotriene metabolic process; response to drug; transmembrane transport; transport; vitamin metabolic process; water-soluble vitamin metabolic process