Apoptosis Associated Tyrosine Kinase (AATK) Recombinant Protein | AATK recombinant protein

Recombinant Apoptosis Associated Tyrosine Kinase (AATK)

Cat. Num. AAA2018813

• Gene Names: Aatk; AATYK; aatyk1; mKIAA0641
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Apoptosis Associated Tyrosine Kinase (AATK); Recombinant Apoptosis Associated Tyrosine Kinase (AATK); AATK recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- YLKE IGHGWFGKVF LGEVHSGVSG TQVVVKELKV SASVQEQMQF LEEAQPYRAL QHSNLLQCLA QCAEVTPYLL VMEFCPLGDL KGYLRSCRVT ESMAPDPLTL QRMACEVACG VLHLHRHNYV HSDLALRNCL LTADLTVKVG DYGLSHCKYR EDYLVTADQL WVPLRWIAPE LVDEVHGNLL VVDQTKSSNV WSLGVTIWEL FELGAQPYPQ HSDRQVLAYA VREQQLKLPK PQLQLALSDR WYEVMQ
• Sequence Length: 1374

• Applicable Applications for AATK recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Tyr137~Gln386 (Accession # Q80YE4) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Membrane; Single-pass type I membrane protein. Cytoplasm. Perinuclear region. Cell projection, dendrite. Axon. Growth cone
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.3
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 311771671
• NCBI GeneID: 11302
• NCBI Accession #: NP_001185714.1
• NCBI GenBank Nucleotide #: NM_001198785.1
• UniProt Accession #: Q80YE4; O35211; Q3U2U5; Q3UHR8; Q66JN3; Q80YE3; Q8CB63; Q8CHE2; A6BLY8
• Molecular Weight: 32.3kDa
• NCBI Official Full Name: serine/threonine-protein kinase LMTK1 isoform 2
• NCBI Official Synonym Full Names: apoptosis-associated tyrosine kinase
• NCBI Official Symbol: Aatk
• NCBI Official Synonym Symbols: AATYK; aatyk1; mKIAA0641
• NCBI Protein Information: serine/threonine-protein kinase LMTK1; lemur tyrosine kinase 1; apoptosis-associated tyrosine kinase 1B; brain apoptosis-associated tyrosine kinase
• UniProt Protein Name: Serine/threonine-protein kinase LMTK1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: Aatk
• UniProt Synonym Gene Names: Aatyk; Kiaa0641; Lmr1; Lmtk1; AATYK
• UniProt Entry Name: LMTK1_MOUSE

Uniprot Description

Lmr1: a protein kinase kinase of the Lmr family. Predominantly expressed in the nervous system and is involved in neurite extension and apoptosis of cerebellar granule cells. Contains a tyrosine kinase domain at the N-terminal end and a proline-rich domain at the C-terminal end. May be necessary for the induction of growth arrest and/or apoptosis of myeloid cells induced by cytokine withdrawal, such as IL3, and during G-CSF-induced terminal differentiation of myeloblasts to granulocytes. Four alternatively spliced isoforms have been reported.

Protein type: Membrane protein, integral; Kinase, protein; Protein kinase, tyrosine (receptor); Protein kinase, TK; EC 2.7.11.1; TK group; Lmr family

Cellular Component: recycling endosome; cell projection; membrane; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; integral to membrane; intracellular

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: neuron apoptosis; apoptosis; protein amino acid autophosphorylation; negative regulation of axon extension; brain development; Rab protein signal transduction; phosphorylation; protein amino acid phosphorylation