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Mouse alpha-2-macroglobulin ELISA Kit | A2M elisa kit

Mouse Alpha-2-macroglobulin, A2M ELISA Kit

Gene Names
Pzp; A1m; A2m; MAM; AI893533
Reactivity
Mouse
Synonyms
alpha-2-macroglobulin; Mouse Alpha-2-macroglobulin; A2M ELISA Kit; Mouse Alpha-2-macroglobulin (A2M) ELISA kit; CPAMD5; DKFZp779B086; FWP007; S863-7; ; A2M elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse A2M. No significant cross-reactivity or interference between Mouse A2M and analogues was observed.
Sequence Length
1495
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for A2M elisa kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with A2M. Standards or samples are then added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) -conjugated antibody preparation specific for A2M, mix well and incubated. The more the amount of A2M in samples, the less HRP- conjugated antibody preparation specific for A2M bound by pre-coated A2M. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. And the color develops in opposite to the amount of A2M in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
165,853 Da
NCBI Official Full Name
alpha-2-macroglobulin
NCBI Official Synonym Full Names
pregnancy zone protein
NCBI Official Symbol
Pzp
NCBI Official Synonym Symbols
A1m; A2m; MAM; AI893533
NCBI Protein Information
alpha-2-macroglobulin; alpha-2-M; alpha 1 macroglobulin
UniProt Protein Name
Alpha-2-macroglobulin
Protein Family
UniProt Gene Name
A2m
UniProt Synonym Gene Names
Pzp; Alpha-2-M
UniProt Entry Name
A2M_MOUSE

Uniprot Description

PZP: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protease inhibitor activity; endopeptidase inhibitor activity; protein complex binding

Biological Process: negative regulation of peptidase activity; embryo implantation

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Product Notes

The Mouse A2M a2m (Catalog #AAA900336) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA900336 ELISA Kit recognizes Mouse A2M. It is sometimes possible for the material contained within the vial of "alpha-2-macroglobulin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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