6-Phosphofructokinase (PFK) Activity Assay Kit | PFK assay kit
6-Phosphofructokinase (PFK) Activity Colorimetric Assay Kit
Synonyms
6-Phosphofructokinase (PFK) Activity; 6-Phosphofructokinase (PFK) Activity Colorimetric Assay Kit; 6-Phosphofructokinase; PFK; Colorimetric; PFK Assay; PFK assay kit
Tag
PFK
Features & Benefits
Detect PFK activity in bacterial supernatant of serum (plasma) animal and plant tissues/cells
Detailed sample preparation and results calculation methods are provided
6-Phosphofructokinase(PFK Activity Colorimetric Assay Kit provides a simple, convenient, rapid and colorimetric PFK detection method. PFK catalyzed fructosetose 6-phosphate and ATP to generate fructose-1, 6-diphosphate and ADP, and pyruvate kinase and lactate dehydrogenase further catalyzed NADH oxidation to NAD+ in turn. The NADH decline rate was measured at 340nm, which reflected the PFK activity
Detailed sample preparation and results calculation methods are provided
6-Phosphofructokinase(PFK Activity Colorimetric Assay Kit provides a simple, convenient, rapid and colorimetric PFK detection method. PFK catalyzed fructosetose 6-phosphate and ATP to generate fructose-1, 6-diphosphate and ADP, and pyruvate kinase and lactate dehydrogenase further catalyzed NADH oxidation to NAD+ in turn. The NADH decline rate was measured at 340nm, which reflected the PFK activity
Kit Components
Extraction Buffer
Assay Buffer
Substrate Mix
Enzyme 1
Enzyme 2
Assay Buffer
Substrate Mix
Enzyme 1
Enzyme 2
Usage Notes
Do not mix or substitute reagents or materials from other kit lots or vendors.
It is advisable to measure serum potassium as soon as possible after blood collection.
Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
It is advisable to measure serum potassium as soon as possible after blood collection.
Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
Preparation and Storage
Store at 4 degree C and protect from light upon receipt. Kit has a storage time of 12 months form receipt.
Shipping||Gel pack with blue ice.
Shipping||Gel pack with blue ice.
Related Product Information for PFK assay kit
Description: 6-Phosphofructokinase(PFK) Colorimetric Assay Kit provides a simple and easy colorimetric assay for measuring PFK in samples.
Background: Phosphofructokinase (PFK) is widely found in animals, plants, microorganisms and cultured cells. It is responsible for the conversion of fructosae 6-phosphate and ATP into fructosae 1,6 diphosphate and ADP. It is one of the key regulatory enzymes in the glycolysis process. Phosphofructokinase (PFK) activity in bacterial cell supernatant and other samples
Background: Phosphofructokinase (PFK) is widely found in animals, plants, microorganisms and cultured cells. It is responsible for the conversion of fructosae 6-phosphate and ATP into fructosae 1,6 diphosphate and ADP. It is one of the key regulatory enzymes in the glycolysis process. Phosphofructokinase (PFK) activity in bacterial cell supernatant and other samples
Product Categories/Family for PFK assay kit
Similar Products
Product Notes
The PFK (Catalog #AAA9719203) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "6-Phosphofructokinase (PFK) Activity, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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