AbFluor 555-Phalloidin Cell Staining
AbFluor 555-Phalloidin
Proprietary AbFluor 555-Phalloidin (Orange Fluorescence)(Ex/Em = 555/565nm)-high-affinity probe for F-actins and much higher photostability than the other fluorescein-phalloidin conjugates.
AbFluor 555-Phalloidin selectively bound to F-actins
it is much higher photostability than the fluorescein-phalloidin conjugates. Phalloidin derivatives are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, which can stain F-actins in cell cultures or cell-free experiments at nanomolar levels.
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Background: Phalloidin belongs to a class of toxins called phallotoxins, which are isolated from the death cap mushroom (Amanita phalloides). It is a bicyclic peptide that binds to F-actin specifically. Therefore, the distribution of F-actin can be very conveniently studied by using a fluorescent dye-labeled phalloidin. Inside the phalloidin, there is an unusual thioether bridge between cysteine and tryptophan, which can form an inner ring structure. When the pH is raised, the thioether is cleaved and the phalloidin loses its affinity for actin.
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Product Notes
The AbFluor 555-Phalloidin (Catalog #AAA9719185) is a Cell Staining and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "AbFluor 555-Phalloidin, Cell Staining" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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