Testing Data
(Fig.1. SIM imaging results of U2OS cells using MyBioSource TraKine Pro Live-cell Tubulin Staining kit (Green Fluorescence with Super Resolution). It can be seen that the kit can specifically label microtubule structures in living cells and perform ultra-high resolution imaging.)
Pro Live-Cell Tubulin Staining Kit
Pro Live-Cell Tubulin Staining Kit (Green Fluorescence with Super Resolution)
Synonyms
Pro Live-Cell Tubulin; Pro Live-Cell Tubulin Staining Kit (Green Fluorescence with Super Resolution); Pro Live-Cell Tubulin staining kit
Features & Benefits
Optimized staining protocol for labeling Tubulin in mammalian living cells.
Especially suitable for Confocal and long-term super-resolution imaging (such as SIM, STED, TIRF, STORM and PALM).
Proprietary TubGreen (Ex/Em = 500/520 nm)-high specificity, low background and excellent photostability.
Low levels of cytotoxicity.
Especially suitable for Confocal and long-term super-resolution imaging (such as SIM, STED, TIRF, STORM and PALM).
Proprietary TubGreen (Ex/Em = 500/520 nm)-high specificity, low background and excellent photostability.
Low levels of cytotoxicity.
Usage Notes
Make sure the pipette tips and PCR tubes were sterilized at high temperature and pressure. Make sure sterile environment and protect from light during the whole experiment.
Preparation and Storage
Refer to list of materials supplied for storage conditions of individual components. Stable for at least 6 months at recommended temperature from date of shipment.
Testing Data
(Fig.1. SIM imaging results of U2OS cells using MyBioSource TraKine Pro Live-cell Tubulin Staining kit (Green Fluorescence with Super Resolution). It can be seen that the kit can specifically label microtubule structures in living cells and perform ultra-high resolution imaging.)
Testing Data
(Fig.1. SIM imaging results of U2OS cells using MyBioSource TraKine Pro Live-cell Tubulin Staining kit (Green Fluorescence with Super Resolution). It can be seen that the kit can specifically label microtubule structures in living cells and perform ultra-high resolution imaging.)
Testing Data
(Fig.2. SIM imaging results of U2OS cells using MyBioSource TraKine Pro Live-cell Tubulin Staining kit (Green Fluorescence with Super Resolution). It can be seen that the kit can specifically label microtubule structures in living cells and perform ultra-high resolution imaging.)
Testing Data
(Fig.2. SIM imaging results of U2OS cells using MyBioSource TraKine Pro Live-cell Tubulin Staining kit (Green Fluorescence with Super Resolution). It can be seen that the kit can specifically label microtubule structures in living cells and perform ultra-high resolution imaging.)
Testing Data
(Fig.3. Confocal imaging results of U2OS cells using MyBioSource TraKine Pro Live-cell Tubulin Staining kit (Green Fluorescence with Super Resolution). It shows that the kit has high labeling efficiency.)
Testing Data
(Fig.3. Confocal imaging results of U2OS cells using MyBioSource TraKine Pro Live-cell Tubulin Staining kit (Green Fluorescence with Super Resolution). It shows that the kit has high labeling efficiency.)
Related Product Information for Pro Live-Cell Tubulin staining kit
Description: This Pro Live-cell Tubulin Staining kit (Green Fluorescence with Super Resolution) is a fluorescence imaging tool for staining of Tubulin in mammalian living cells with high specificity and low background. The proprietary probe in the kit consists of a microtubule recognition unit and a green fluorescent dye (Ex/Em=500/520 nm), the recognition unit can selectively recognize microtubules and binding with it. The whole probe is membrane impermeable, but after incubating with buffer T, it can enter cells through endocytosis pathway in vesicular compartments, and release into cytoplasm subsequently. It is especially suitable for long-term super-resolution imaging.
Background: Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. Tubulin is a heterodimer, which consists of a-tubulin and b-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology. The most studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure.
Background: Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. Tubulin is a heterodimer, which consists of a-tubulin and b-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology. The most studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure.
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Product Notes
The Pro Live-Cell Tubulin (Catalog #AAA9718801) is a Staining Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Pro Live-Cell Tubulin, Staining Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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