FGF19 recombinant protein

Human FGF19 Protein

Cat. Num. AAA9718712

• Purity: >97% as determined by SDS-PAGE
• Synonyms: FGF19; Human FGF19 Protein; Recombinant Human FGF19 Protein (Fibroblast growth factor 19) expressed in E. coli; Fibroblast growth factor 19; FGF19 recombinant protein

• Host: E Coli
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 15% gly, 50mM Tris, 2mM EDTA
• Sequence: Amino acid sequence derived from mature form of human FGF19 (O95750) (Phe27-Lys216) was expressed, with a N-terminal Met.
• Sequence Length: 216

• Species: Human
• Protein Length: The recombinant human FGF19 consists of 191 amino acids and predicts a molecular mass of 21.4 KDa. It migrates as an approximately 23 KDa band in SDS-PAGE under reducing conditions.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human FGF19 protein in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Item should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Human FGF19 protein.)

Related Product Information for FGF19 recombinant protein

FGF19, also known as FGF-19, is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF19 interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by KL, KLB and heparan sulfate glycosaminoglycans that function as coreceptors. It interacts with KL and KLB directly. However, it interacts with FGFR4 in the presence of heparin, KL or KLB. FGF19 is involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. It also stimulates glucose uptake in adipocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 4826726
• NCBI GeneID: 9965
• NCBI Accession #: NP_005108.1
• NCBI GenBank Nucleotide #: NP_005108.1
• UniProt Accession #: O95750
• Molecular Weight: 21.4 kDa
• NCBI Official Full Name: fibroblast growth factor 19
• NCBI Official Synonym Full Names: fibroblast growth factor 19
• NCBI Official Symbol: FGF19
• NCBI Protein Information: fibroblast growth factor 19
• UniProt Protein Name: Fibroblast growth factor 19
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF19
• UniProt Synonym Gene Names: FGF-19
• UniProt Entry Name: FGF19_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. This growth factor is a high affinity, heparin dependent ligand for FGFR4. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF19: Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4. Belongs to the heparin-binding growth factors family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Cytokine; Secreted; Cell development/differentiation

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: extracellular region

Molecular Function: protein binding; growth factor activity; fibroblast growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; nervous system development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; heart development; positive regulation of JNK cascade; positive regulation of glucose import; positive regulation of cell proliferation; insulin receptor signaling pathway; innate immune response; positive regulation of protein amino acid phosphorylation; neural crest cell migration