IL-8 active protein

Human IL-8 Protein (aa 23-99)

Cat. Num. AAA9718666

• Gene Names: CXCL8; IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: IL-8; Human IL-8 Protein (aa 23-99); Recombinant Human IL8 Protein (aa 23-99) (Interleukin-8) expressed in Human Cells; GCP-1; GCP1; IL8; Interleukin-8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1; CXCL8; IL-8 active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 100mM NaCl, 50mM Tris, pH 7.5
• Sequence: The 77 amino acid endothelial-cell derived form (Ala 23-Ser 99) of the mature human IL8 (NP_000575.1) was expressed and purified.
• Sequence Length: 99

• Application Notes: Others: The endotoxin level is less then 1.0 EU per ug of the protein as determined by the LAL method.
• Species: Human
• Protein Length: The recombinant mature human cytokine IL8 comprising the 1-77 amino acids predicts a molecular mass of 8 kDa, and migrates as an approximately 12 kDa protein in SDS-PAGE due to glycosylation.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL8 protein (aa 23-99) in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Activity: Mesured by its ability to induce IFN-gamma secretion by KG-1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is typically 15-75 ng/mL.
• Preperation Method: HEK293 Cells
• Preparation and Storage: Lyophilized Human IL8 protein (aa 23-99) product should be stored desiccated below -18°C. Upon reconstitution , the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Human IL-8 protein (aa 23-99).)

Related Product Information for IL-8 active protein

Interleukin 8 (IL-8), also known as CXCL8, which is a chemokine with a defining CXC amino acid motif that was initially characterized for its leukocyte chemotactic activity, is now known to possess tumorigenic and proangiogenic properties as well. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli (IL1, TNF, LPS, etc). In human gliomas, IL-8 is expressed and secreted at high levels both in vitro and in vivo, and recent experiments suggest it is critical to glial tumor neovascularity and progression. Levels of IL-8 correlate with histologic grade in glial neoplasms, and the most malignant form, glioblastoma, shows the highest expression in pseudopalisading cells around necrosis, suggesting that hypoxia/anoxia may stimulate expression. Interleukin (IL)-8/CXCL8 is a potent neutrophil chemotactic factor. Accumulating evidence has demonstrated that various types of cells can produce a large amount of IL-8/CXCL8 in response to a wide variety of stimuli, including proinflammatory cytokines, microbes and their products, and environmental changes such as hypoxia, reperfusion, and hyperoxia. Numerous observations have established IL-8/CXCL8 as a key mediator in neutrophil-mediated acute inflammation due to its potent actions on neutrophils. However, several lines of evidence indicate that IL-8/CXCL8 has a wide range of actions on various types of cells, including lymphocytes, monocytes, endothelial cells, and fibroblasts, besides neutrophils. The discovery of these biological functions suggests that IL-8/CXCL8 has crucial roles in various pathological conditions such as chronic inflammation and cancer. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.

NCBI and Uniprot Product Information

• NCBI GI #: 10834978
• NCBI GeneID: 3576
• NCBI Accession #: NP_000575.1
• NCBI GenBank Nucleotide #: NP_000575.1
• UniProt Accession #: P10145
• Molecular Weight: 8kDa
• NCBI Official Full Name: interleukin-8 isoform 1
• NCBI Official Synonym Full Names: C-X-C motif chemokine ligand 8
• NCBI Official Symbol: CXCL8
• NCBI Official Synonym Symbols: IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
• NCBI Protein Information: interleukin-8

NCBI Description

The protein encoded by this gene is a member of the CXC chemokine family and is a major mediator of the inflammatory response. The encoded protein is secreted primarily by neutrophils, where it serves as a chemotactic factor by guiding the neutrophils to the site of infection. This chemokine is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other members of the CXC chemokine gene family form a gene cluster in a region of chromosome 4q. [provided by RefSeq, Aug 2017]