Mouse DNA-Directed RNA Polymerase III Subunit RPC6 (POLR3F) ELISA Kit | POLR3F elisa kit

Mouse DNA-Directed RNA Polymerase III Subunit RPC6 (POLR3F) ELISA Kit

Cat. Num. AAA9718051

• Gene Names: Polr3f; RPC6; RPC39; 2810411G20Rik; 3010019O03Rik; 3110032A07Rik
• Reactivity: Mouse
• Synonyms: DNA-Directed RNA Polymerase III Subunit RPC6 (POLR3F); Mouse DNA-Directed RNA Polymerase III Subunit RPC6 (POLR3F) ELISA Kit; POLR3F; MGC13517; RPC39; RPC6; DNA-directed RNA polymeraseI 39 kDa polypeptide F; DNA-directed RNA polymerasesI 39 kDa polypeptide; RNA polymeraseI C39 subunit; POLR3F elisa kit

• Reactivity: Mouse
• Specificity: Mouse DNA-directed RNA polymerase III subunit RPC6 (POLR3F) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse POLR3F. No significant cross-reactivity or interference between Mouse POLR3F and analogues was observed.
• Sequence Length: 316

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse DNA-directed RNA polymerase III subunit RPC6 (POLR3F) Standard Curve.)

Related Product Information for POLR3F elisa kit

Description: This Mouse DNA-directed RNA polymerase III subunit RPC6 (POLR3F) ELISA Kit employs a two-site sandwich ELISA to quantitate POLR3F in samples. An antibody specific for POLR3F has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any POLR3F present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLR3F is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLR3F bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: POLR3F is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. hRPC39 interacts physically with both hTBP and hTFIIIB90, two subunits of human RNA Pol III transcription initiation factor IIIB. These data strongly suggest that the hRPC32-hRPC39-hRPC62 subcomplex directs RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and hRPC39.

NCBI and Uniprot Product Information

• NCBI GI #: 27754160
• NCBI GeneID: 70408
• NCBI Accession #: NP_084039.2
• NCBI GenBank Nucleotide #: NP_084039.2
• UniProt Accession #: Q921X6
• NCBI Official Full Name: DNA-directed RNA polymerase III subunit RPC6
• NCBI Official Synonym Full Names: polymerase (RNA) III (DNA directed) polypeptide F
• NCBI Official Symbol: Polr3f
• NCBI Official Synonym Symbols: RPC6; RPC39; 2810411G20Rik; 3010019O03Rik; 3110032A07Rik
• NCBI Protein Information: DNA-directed RNA polymerase III subunit RPC6
• UniProt Protein Name: DNA-directed RNA polymerase III subunit RPC6
• UniProt Gene Name: Polr3f
• UniProt Synonym Gene Names: RNA polymerase III subunit C6

Uniprot Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct RNA Pol III binding to the TFIIIB-DNA complex. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway (). Preferentially binds double-stranded DNA (dsDNA) ().