Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Peripherin (PRPH) Standard Curve.)
Mouse Peripherin (PRPH) ELISA Kit | PRPH elisa kit
Mouse Peripherin (PRPH) ELISA Kit
Gene Names
Prph; Prph1
Reactivity
Mouse
Synonyms
Peripherin (PRPH); Mouse Peripherin (PRPH) ELISA Kit; PRPH; NEF4; PRPH1; neurofilament 4 (57kD); PRPH elisa kit
Reactivity
Mouse
Specificity
Mouse Peripherin (PRPH) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRPH. No significant cross-reactivity or interference between Mouse PRPH and analogues was observed.
Sequence Length
475
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Peripherin (PRPH) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Peripherin (PRPH) Standard Curve.)
Related Product Information for PRPH elisa kit
Description: This Mouse Peripherin (PRPH) ELISA Kit employs a two-site sandwich ELISA to quantitate PRPH in samples. An antibody specific for PRPH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRPH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRPH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRPH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Peripherin, a type III intermediate filament protein, was initially described by Portier et al. (1984) as a cytoskeletal protein present in neurons of the mammalian peripheral nervous system (hence, the name) and in cultured neuroblastoma cells. The amino-terminal region contained potential serine phosphorylation sites. Northern blot analysis detected a corresponding mRNA in ganglia of the peripheral nervous system, including the superior cervical ganglion, ciliary ganglion, and dorsal root ganglion. In the central nervous system, expression was detected in motor nuclei of cranial nerves and ventral horn neurons. Expression was induced by nerve growth factor.
Background: Peripherin, a type III intermediate filament protein, was initially described by Portier et al. (1984) as a cytoskeletal protein present in neurons of the mammalian peripheral nervous system (hence, the name) and in cultured neuroblastoma cells. The amino-terminal region contained potential serine phosphorylation sites. Northern blot analysis detected a corresponding mRNA in ganglia of the peripheral nervous system, including the superior cervical ganglion, ciliary ganglion, and dorsal root ganglion. In the central nervous system, expression was detected in motor nuclei of cranial nerves and ventral horn neurons. Expression was induced by nerve growth factor.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Peripherin
NCBI Official Synonym Full Names
peripherin
NCBI Official Symbol
Prph
NCBI Official Synonym Symbols
Prph1
NCBI Protein Information
peripherin
UniProt Protein Name
Peripherin
Protein Family
UniProt Gene Name
Prph
UniProt Synonym Gene Names
Prph1
UniProt Entry Name
PERI_MOUSE
Research Articles on PRPH
Similar Products
Product Notes
The Mouse PRPH prph (Catalog #AAA9718040) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718040 ELISA Kit recognizes Mouse PRPH. It is sometimes possible for the material contained within the vial of "Peripherin (PRPH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
