Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Ribonuclease-like protein 13 (RNASE13) Standard Curve.)
Mouse Ribonuclease-Like Protein 13 (RNASE13) ELISA Kit | RNASE13 elisa kit
Mouse Ribonuclease-Like Protein 13 (RNASE13) ELISA Kit
Reactivity
Mouse
Synonyms
Ribonuclease-Like Protein 13 (RNASE13); Mouse Ribonuclease-Like Protein 13 (RNASE13) ELISA Kit; RNASE13; Ribonuclease 13; ribonuclease; RNase A family; 13; RNASE13 elisa kit
Reactivity
Mouse
Specificity
Mouse Ribonuclease-like protein 13 (RNASE13) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse RNASE13. No significant cross-reactivity or interference between Mouse RNASE13 and analogues was observed.
Sequence Length
153
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Ribonuclease-like protein 13 (RNASE13) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Ribonuclease-like protein 13 (RNASE13) Standard Curve.)
Related Product Information for RNASE13 elisa kit
Description: This Mouse Ribonuclease-like protein 13 (RNASE13) ELISA Kit employs a two-site sandwich ELISA to quantitate RNASE13 in samples. An antibody specific for RNASE13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RNASE13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RNASE13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RNASE13 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: RNASE13 belongs to the pancreatic ribonuclease family. Ribonuclease A (RNase A) is a pancreatic ribonuclease that cleaves single-stranded RNA. Bovine pancreatic RNase A is one of the classic model systems of protein science. RNase A is a relatively small protein. It can be characterized as a two-layer alpha + beta protein that is folded in half to resemble a taco, with a deep cleft for binding the RNA substrate. The first layer is composed of three alpha helices from the N-terminal half of the protein. The second layer consist of three beta-hairpins arranged in two beta-sheets. The hairpins 61-74 and 105-124 form a four-stranded, antiparallel beta-sheet that lies on helix 3. The longest beta-hairpin 79-104 mates with a short beta-strand to form a three-stranded, antiparallel beta-sheet that lies on helix 2.
Background: RNASE13 belongs to the pancreatic ribonuclease family. Ribonuclease A (RNase A) is a pancreatic ribonuclease that cleaves single-stranded RNA. Bovine pancreatic RNase A is one of the classic model systems of protein science. RNase A is a relatively small protein. It can be characterized as a two-layer alpha + beta protein that is folded in half to resemble a taco, with a deep cleft for binding the RNA substrate. The first layer is composed of three alpha helices from the N-terminal half of the protein. The second layer consist of three beta-hairpins arranged in two beta-sheets. The hairpins 61-74 and 105-124 form a four-stranded, antiparallel beta-sheet that lies on helix 3. The longest beta-hairpin 79-104 mates with a short beta-strand to form a three-stranded, antiparallel beta-sheet that lies on helix 2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
probable inactive ribonuclease-like protein 13
NCBI Official Synonym Full Names
ribonuclease, RNase A family, 13 (non-active)
NCBI Official Symbol
Rnase13
NCBI Protein Information
probable inactive ribonuclease-like protein 13
UniProt Protein Name
Probable inactive ribonuclease-like protein 13
Protein Family
UniProt Gene Name
Rnase13
UniProt Entry Name
RNS13_MOUSE
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Product Notes
The Mouse RNASE13 rnase13 (Catalog #AAA9718033) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718033 ELISA Kit recognizes Mouse RNASE13. It is sometimes possible for the material contained within the vial of "Ribonuclease-Like Protein 13 (RNASE13), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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