Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Crystallin, beta A1 (CRYBA1) Standard Curve.)
Mouse Crystallin, beta A1 (CRYBA1) ELISA Kit | CRYBA1 elisa kit
Mouse Crystallin, beta A1 (CRYBA1) ELISA Kit
Reactivity
Mouse
Synonyms
Crystallin; beta A1 (CRYBA1); Mouse Crystallin; beta A1 (CRYBA1) ELISA Kit; CRYBA1; CRYB1; crystallin; beta A3; eye lens structural protein; CRYBA1 elisa kit
Reactivity
Mouse
Specificity
Mouse Crystallin, beta A1 (CRYBA1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse CRYBA1. No significant cross-reactivity or interference between Mouse CRYBA1 and analogues was observed.
Sequence Length
198
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Crystallin, beta A1 (CRYBA1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Crystallin, beta A1 (CRYBA1) Standard Curve.)
Related Product Information for CRYBA1 elisa kit
Description: This Mouse Crystallin, beta A1 (CRYBA1) ELISA Kit employs a two-site sandwich ELISA to quantitate CRYBA1 in samples. An antibody specific for CRYBA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CRYBA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CRYBA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CRYBA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. CRYbA1, a beta acidic group member, encodes two proteins (crystallin, beta A3 and crystallin, beta A1) from a single mRNA, the latter protein is 17 aa shorter than crystallin, beta A3 and is generated by use of an alternate translation initiation site. Deletion of exons 3 and 4 causes the autosomal dominant disease 'zonular cataract with sutural opacities'.
Background: Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. CRYbA1, a beta acidic group member, encodes two proteins (crystallin, beta A3 and crystallin, beta A1) from a single mRNA, the latter protein is 17 aa shorter than crystallin, beta A3 and is generated by use of an alternate translation initiation site. Deletion of exons 3 and 4 causes the autosomal dominant disease 'zonular cataract with sutural opacities'.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
NCBI Official Full Name
Beta-crystallin A1
UniProt Protein Name
Beta-crystallin A1
Protein Family
UniProt Gene Name
Cryba1
Uniprot Description
Crystallins are the dominant structural components of the vertebrate eye lens.
Similar Products
Product Notes
The Mouse CRYBA1 cryba1 (Catalog #AAA9718007) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718007 ELISA Kit recognizes Mouse CRYBA1. It is sometimes possible for the material contained within the vial of "Crystallin, beta A1 (CRYBA1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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