Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Left-right determination factor 1 (LEFTY1) Standard Curve.)
Mouse Left-Right Determination Factor 1 (LEFTY1) ELISA Kit | LEFTY1 elisa kit
Mouse Left-Right Determination Factor 1 (LEFTY1) ELISA Kit
Gene Names
Lefty1; Ebaf; Leftb; Stra3; Tgfb4; lefty; lefty-1; AI450052
Reactivity
Mouse
Synonyms
Left-Right Determination Factor 1 (LEFTY1); Mouse Left-Right Determination Factor 1 (LEFTY1) ELISA Kit; LEFTY1; LEFTB; LEFTYB; left-right determination; factor B; LEFTY1 elisa kit
Reactivity
Mouse
Specificity
Mouse Left-right determination factor 1 (LEFTY1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LEFTY1. No significant cross-reactivity or interference between Mouse LEFTY1 and analogues was observed.
Sequence Length
368
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Left-right determination factor 1 (LEFTY1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Left-right determination factor 1 (LEFTY1) Standard Curve.)
Related Product Information for LEFTY1 elisa kit
Description: This Mouse Left-right determination factor 1 (LEFTY1) ELISA Kit employs a two-site sandwich ELISA to quantitate LEFTY1 in samples. An antibody specific for LEFTY1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LEFTY1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LEFTY1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LEFTY1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Vertebrates exhibit numerous left-right (L-R) asymmetries, such as the position of the heart and spleen on the left side. L-R polarity is determined early during development, around the presomite stage in mammals. Several genes have been shown to be expressed asymmetrically relative to the L-R axis. Meno et al. (1996, 1997) identified 2 transforming growth factor-beta-related genes, Lefty1 and Lefty2, that are expressed on the left side of developing mouse embryos.Kosaki et al. (1999) characterized 2 homologs of the murine Lefty1 and Lefty2 genes, which they designated LEFTY B and LEFTY A, respectively. Both genes encode proteins with 366 amino acids. The deduced amino acid sequences of LEFTY B and LEFTY A are more similar to each other than to mouse Lefty1 or Lefty2.
Background: Vertebrates exhibit numerous left-right (L-R) asymmetries, such as the position of the heart and spleen on the left side. L-R polarity is determined early during development, around the presomite stage in mammals. Several genes have been shown to be expressed asymmetrically relative to the L-R axis. Meno et al. (1996, 1997) identified 2 transforming growth factor-beta-related genes, Lefty1 and Lefty2, that are expressed on the left side of developing mouse embryos.Kosaki et al. (1999) characterized 2 homologs of the murine Lefty1 and Lefty2 genes, which they designated LEFTY B and LEFTY A, respectively. Both genes encode proteins with 366 amino acids. The deduced amino acid sequences of LEFTY B and LEFTY A are more similar to each other than to mouse Lefty1 or Lefty2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
left-right determination factor 1 preproprotein
NCBI Official Synonym Full Names
left right determination factor 1
NCBI Official Symbol
Lefty1
NCBI Official Synonym Symbols
Ebaf; Leftb; Stra3; Tgfb4; lefty; lefty-1; AI450052
NCBI Protein Information
left-right determination factor 1
UniProt Protein Name
Left-right determination factor 1
Protein Family
UniProt Gene Name
Lefty1
UniProt Synonym Gene Names
Ebaf; Lefty; Stra3; Tgfb4; Lefty protein; TGF-beta-4
NCBI Description
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in left-right asymmetry determination of organ systems during development. Mice lacking a functional copy of this gene exhibit embryonic lethality and defects in left-right patterning. [provided by RefSeq, Aug 2016]
Uniprot Description
Required for left-right axis determination as a regulator of LEFTY2 and NODAL.
Research Articles on LEFTY1
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Product Notes
The Mouse LEFTY1 lefty1 (Catalog #AAA9717796) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717796 ELISA Kit recognizes Mouse LEFTY1. It is sometimes possible for the material contained within the vial of "Left-Right Determination Factor 1 (LEFTY1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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