Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Cartilage matrix protein (MATN1) Standard Curve.)
Mouse Cartilage Matrix Protein (MATN1) ELISA Kit | CMP elisa kit
Mouse Cartilage Matrix Protein (MATN1) ELISA Kit
Gene Names
Matn1; CMP; Crtm; Mat1; matrilin-1
Reactivity
Mouse
Synonyms
Cartilage Matrix Protein (MATN1); Mouse Cartilage Matrix Protein (MATN1) ELISA Kit; MATN1; CRTM; cartilage matrix protein; CMP elisa kit
Reactivity
Mouse
Specificity
Mouse Cartilage matrix protein (MATN1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MATN1. No significant cross-reactivity or interference between Mouse MATN1 and analogues was observed.
Sequence Length
500
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Cartilage matrix protein (MATN1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Cartilage matrix protein (MATN1) Standard Curve.)
Related Product Information for CMP elisa kit
Description: This Mouse Cartilage matrix protein (MATN1) ELISA Kit employs a two-site sandwich ELISA to quantitate MATN1 in samples. An antibody specific for MATN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MATN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MATN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MATN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MATN1 is a member of von Willebrand factor A domain containing protein family. This family of proteins are thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. Mutations of this gene have been associated with variety of inherited chondrodysplasias.Three microsatellite polymorphisms in the gene, respectively consisting of 103 bp, 101 bp and 99 bp, have been linked to idiopathic scoliosis.The genomic probe was also used to screen a human retina cDNA library. The protein sequence predicted by the cDNA clones has 496 amino acids, including a 22-residue signal peptide. The structure of the CMP gene (also symbolized CRTM) and polypeptide were strikingly similar in the chicken and in the human. The human gene spans 12 kb and has 8 exons and 7 introns.
Background: MATN1 is a member of von Willebrand factor A domain containing protein family. This family of proteins are thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. Mutations of this gene have been associated with variety of inherited chondrodysplasias.Three microsatellite polymorphisms in the gene, respectively consisting of 103 bp, 101 bp and 99 bp, have been linked to idiopathic scoliosis.The genomic probe was also used to screen a human retina cDNA library. The protein sequence predicted by the cDNA clones has 496 amino acids, including a 22-residue signal peptide. The structure of the CMP gene (also symbolized CRTM) and polypeptide were strikingly similar in the chicken and in the human. The human gene spans 12 kb and has 8 exons and 7 introns.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cartilage matrix protein
NCBI Official Synonym Full Names
matrilin 1, cartilage matrix protein
NCBI Official Symbol
Matn1
NCBI Official Synonym Symbols
CMP; Crtm; Mat1; matrilin-1
NCBI Protein Information
cartilage matrix protein
UniProt Protein Name
Cartilage matrix protein
UniProt Gene Name
Matn1
UniProt Synonym Gene Names
Cmp; Crtm
Uniprot Description
MATN1: Cartilage matrix protein is a major component of the extracellular matrix of non-articular cartilage. It binds to collagen.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 4 D2.2|4 64.09 cM
Cellular Component: extracellular region; proteinaceous extracellular matrix
Molecular Function: calcium ion binding; extracellular matrix structural constituent; protein binding
Biological Process: chondrocyte differentiation; regulation of bone mineralization
Research Articles on CMP
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Product Notes
The Mouse CMP matn1 (Catalog #AAA9717721) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717721 ELISA Kit recognizes Mouse CMP. It is sometimes possible for the material contained within the vial of "Cartilage Matrix Protein (MATN1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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