Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Mannose-binding protein C (MBL2) Standard Curve.)
Mouse Mannose-Binding Protein C (MBL2) ELISA Kit | MBP elisa kit
Mouse Mannose-Binding Protein C (MBL2) ELISA Kit
Gene Names
Mbl2; MBL; L-MBP; MBL-C; MBP-C; RARF/P28A
Reactivity
Mouse
Synonyms
Mannose-Binding Protein C (MBL2); Mouse Mannose-Binding Protein C (MBL2) ELISA Kit; MBL2; COLEC1; HSMBPC; MBL; MBP1; MGC116832; MGC116833; mannan-binding lectin; mannose-binding lectin 2; soluble (opsonic defect); mannose-binding protein C; soluble mannose-binding lectin; MBP elisa kit
Reactivity
Mouse
Specificity
Mouse Mannose-binding protein C (MBL2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MBL2. No significant cross-reactivity or interference between Mouse MBL2 and analogues was observed.
Sequence Length
244
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Mannose-binding protein C (MBL2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Mannose-binding protein C (MBL2) Standard Curve.)
Related Product Information for MBP elisa kit
Description: This Mouse Mannose-binding protein C (MBL2) ELISA Kit employs a two-site sandwich ELISA to quantitate MBL2 in samples. An antibody specific for MBL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MBL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MBL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MBL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Mannosebinding protein (MBP), initiates the lectin branch of the innate immune response by binding to the surface of potentially pathogenic microorganisms and initiating complement fixation through an N-terminal collagen-like domain. MBL is a key component in immune response in that it can directly trigger neutralization of invading microorganisms by an Ab-independent mechanism.It binds to sugars on the surface of bacterial, fungal and parasitic cells through C-terminal, Ca2 -dependent carbohydrate-recognition domains. Mutations of human MBL are associated with immunodeficiency resulting from a reduction in the ability of the mutant MBL to initiate complement fixation.
Background: Mannosebinding protein (MBP), initiates the lectin branch of the innate immune response by binding to the surface of potentially pathogenic microorganisms and initiating complement fixation through an N-terminal collagen-like domain. MBL is a key component in immune response in that it can directly trigger neutralization of invading microorganisms by an Ab-independent mechanism.It binds to sugars on the surface of bacterial, fungal and parasitic cells through C-terminal, Ca2 -dependent carbohydrate-recognition domains. Mutations of human MBL are associated with immunodeficiency resulting from a reduction in the ability of the mutant MBL to initiate complement fixation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mannose-binding protein C
NCBI Official Synonym Full Names
mannose-binding lectin (protein C) 2
NCBI Official Symbol
Mbl2
NCBI Official Synonym Symbols
MBL; L-MBP; MBL-C; MBP-C; RARF/P28A
NCBI Protein Information
mannose-binding protein C
UniProt Protein Name
Mannose-binding protein C
Protein Family
UniProt Gene Name
Mbl2
UniProt Synonym Gene Names
MBP-C; RARF/P28A
UniProt Entry Name
MBL2_MOUSE
Uniprot Description
MBL2: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: extracellular space; collagen; extracellular region
Molecular Function: mannose binding; protein self-association; protease binding; galactose binding; calcium ion binding; carbohydrate binding; calcium-dependent protein binding; receptor binding
Biological Process: defense response to Gram-positive bacterium; killing by host of symbiont cells; immune system process; positive regulation of phagocytosis; innate immune response; complement activation, lectin pathway; complement activation, classical pathway; negative regulation of viral reproduction
Research Articles on MBP
Similar Products
Product Notes
The Mouse MBP mbl2 (Catalog #AAA9717713) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717713 ELISA Kit recognizes Mouse MBP. It is sometimes possible for the material contained within the vial of "Mannose-Binding Protein C (MBL2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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