Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse MyoD family inhibitor domain-containing protein (MDFIC) Standard Curve.)
Mouse MyoD Family Inhibitor Domain-Containing Protein (MDFIC) ELISA Kit | MDFIC elisa kit
Mouse MyoD Family Inhibitor Domain-Containing Protein (MDFIC) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse MyoD family inhibitor domain-containing protein (MDFIC) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse MyoD family inhibitor domain-containing protein (MDFIC) Standard Curve.)
Background: Mdfic product is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain, which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG), and an in-frame, downstream AUG codon, results in the production of two isoforms, which in human have been shown to have different subcellular localization.
NCBI and Uniprot Product Information
NCBI Description
This gene product is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain, which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG), and an in-frame, downstream AUG codon, results in the production of two isoforms, which in human have been shown to have different subcellular localization. [provided by RefSeq, Jul 2008]
Uniprot Description
MDFIC: Acts as a transcriptional activator or repressor. Inhibits the transcriptional activation of Zic family proteins ZIC1, ZIC2 and ZIC3. Retains nuclear Zic proteins ZIC1, ZIC2 and ZIC3 in the cytoplasm. Modulates the expression from both cellular and viral promoters. Down-regulates Tat-dependent transcription of the human immunodeficiency virus type 1 (HIV-1) LTR by interacting with HIV-1 Tat and Rev and impairing their nuclear import, probably by rendering the NLS domains inaccessible to importin- beta. Also stimulates activation of human T-cell leukemia virus type I (HTLV-I) LTR. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways. Belongs to the MDFI family. 2 isoforms of the human protein are produced by alternative initiation.
Protein type: Nucleolus
Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; nucleolus; nucleus
Molecular Function: protein binding; cyclin binding; Tat protein binding; transcription factor binding
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of viral transcription; positive regulation of transcription, DNA-dependent; regulation of Wnt receptor signaling pathway; negative regulation of protein import into nucleus; negative regulation of transcription, DNA-dependent; activation of JNK activity
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Product Notes
The Mouse MDFIC mdfic (Catalog #AAA9717697) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717697 ELISA Kit recognizes Mouse MDFIC. It is sometimes possible for the material contained within the vial of "MyoD Family Inhibitor Domain-Containing Protein (MDFIC), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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